Virus-like particle size and molecular weight/mass determination applying gas-phase electrophoresis (native nES GEMMA)

Anal Bioanal Chem. 2019 Sep;411(23):5951-5962. doi: 10.1007/s00216-019-01998-6. Epub 2019 Jul 6.

Abstract

(Bio-)nanoparticle analysis employing a nano-electrospray gas-phase electrophoretic mobility molecular analyzer (native nES GEMMA) also known as nES differential mobility analyzer (nES DMA) is based on surface-dry analyte separation at ambient pressure. Based on electrophoretic principles, single-charged nanoparticles are separated according to their electrophoretic mobility diameter (EMD) corresponding to the particle size for spherical analytes. Subsequently, it is possible to correlate the (bio-)nanoparticle EMDs to their molecular weight (MW) yielding a corresponding fitted curve for an investigated analyte class. Based on such a correlation, (bio-)nanoparticle MW determination via its EMD within one analyte class is possible. Turning our attention to icosahedral, non-enveloped virus-like particles (VLPs), proteinaceous shells, we set up an EMD/MW correlation. We employed native electrospray ionization mass spectrometry (native ESI MS) to obtain MW values of investigated analytes, where possible, after extensive purification. We experienced difficulties in native ESI MS with time-of-flight (ToF) detection to determine MW due to sample inherent characteristics, which was not the case for charge detection (CDMS). nES GEMMA exceeds CDMS in speed of analysis and is likewise less dependent on sample purity and homogeneity. Hence, gas-phase electrophoresis yields calculated MW values in good approximation even when charge resolution was not obtained in native ESI ToF MS. Therefore, both methods-native nES GEMMA-based MW determination via an analyte class inherent EMD/MW correlation and native ESI MS-in the end relate (bio-)nanoparticle MW values. However, they differ significantly in, e.g., ease of instrument operation, sample and analyte handling, or costs of instrumentation. Graphical abstract.

Keywords: DMA; Mass spectrometry; Molecular weight/mass; Native nES GEMMA; Size; VLP.

MeSH terms

  • Electrophoresis / methods*
  • Molecular Weight
  • Particle Size
  • Proteins / chemistry
  • Spectrometry, Mass, Electrospray Ionization / methods*
  • Vaccines, Virus-Like Particle / chemistry*
  • Virion / chemistry
  • Viruses / chemistry*

Substances

  • Proteins
  • Vaccines, Virus-Like Particle