Quantitative immunoassay of anti-La antibodies using purified recombinant La antigen

Arthritis Rheum. 1988 Apr;31(4):506-14. doi: 10.1002/art.1780310407.

Abstract

A purified recombinant La fusion protein was tested in an enzyme-linked immunosorbent assay to quantitate anti-La responses. This protein contained the immunodominant region of the La molecule fused to beta-galactosidase. In solid-phase assays, recombinant La protein was solubilized in urea and bound to polystyrene wells without loss of immunoreactivity. The recombinant-based enzyme-linked immunosorbent assay proved to be a sensitive method for the detection of anti-La binding, and it accurately distinguished anti-La precipitin positive sera from normal sera.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies / analysis*
  • Autoantigens / immunology*
  • Bacterial Proteins / immunology
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme-Linked Immunosorbent Assay
  • Escherichia coli / immunology
  • Humans
  • Ribonucleoproteins*
  • SS-B Antigen
  • beta-Galactosidase / immunology

Substances

  • Antibodies
  • Autoantigens
  • Bacterial Proteins
  • Ribonucleoproteins
  • beta-Galactosidase