Activation of DNA-binding activity in an apparently cytoplasmic precursor of the NF-kappa B transcription factor

Cell. 1988 Apr 22;53(2):211-7. doi: 10.1016/0092-8674(88)90382-0.


In cells that do not express kappa immunoglobulin light chain genes, the kappa enhancer-binding protein NF-kappa B is not evident in either cytoplasmic or nuclear fractions. By denaturation, size fractionation, and renaturation, however, NF-kappa B activity can be revealed in cytosolic fractions, showing that the DNA-binding protein is present but inhibited in its binding activity. By using a variety of protocols involving the dissociating agents sodium desoxycholate and formamide, as much cytosolic NF-kappa B can be found in the fraction from unstimulated 70Z/3 pre-B cells as is found in the nuclear extract from phorbol ester-activated cells. We conclude that both 70Z/3 and HeLa cells contain apparently cytosolic NF-kappa B in a form with no evident DNA-binding activity, and phorbol esters both release the inhibition of binding and cause a translocation to the nucleus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Cell Nucleus / drug effects
  • Cell Nucleus / metabolism
  • Cytosol / immunology
  • Cytosol / metabolism
  • DNA-Binding Proteins / metabolism*
  • Enhancer Elements, Genetic*
  • Genes, Immunoglobulin*
  • HeLa Cells / immunology
  • Humans
  • Immunoglobulin kappa-Chains / genetics*
  • Protein Denaturation
  • Tetradecanoylphorbol Acetate / pharmacology
  • Transcription Factors / metabolism*


  • DNA-Binding Proteins
  • Immunoglobulin kappa-Chains
  • Transcription Factors
  • Tetradecanoylphorbol Acetate