Levels of adrenodoxin, NADPH-cytochrome P-450 reductase and cytochromes P-45011 beta, P-45021, P-450scc, in adrenal zona fasciculata-reticularis tissue from androgen-treated rats

Mol Cell Endocrinol. 1988 Jan;55(1):15-20. doi: 10.1016/0303-7207(88)90086-x.


Treatment of rats with methylandrostenediol (MAD), an anabolic androgen, caused a profound reduction (65%) in the level of cytochrome P-450 11 beta in rat adrenocortical mitochondria as measured by immunoblots using a specific antibody. The decreases in mitochondrial cytochrome P-450scc (15%) and adrenodoxin (20%) were much less than that observed for cytochrome P-45011 beta. A 35% decrease in adrenal microsomal cytochrome P-450 21 and NADPH-cytochrome P-450 reductase levels was brought about by the treatment with MAD. The data establish that the preferential decrease in adrenal steroid 11 beta-hydroxylase activity associated with androgen treatment results from a decrease in cytochrome P-450 11 beta. This is consistent with the role of 11-deoxycorticosterone in the pathogenesis of androgen-induced hypertension in rats.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adrenal Cortex / drug effects
  • Adrenal Cortex / metabolism*
  • Adrenodoxin / metabolism*
  • Androstenediols / pharmacology*
  • Animals
  • Cytochrome P-450 Enzyme System / metabolism*
  • Female
  • Isoenzymes / metabolism*
  • Kinetics
  • Male
  • Methandriol / pharmacology*
  • Mitochondria / drug effects
  • Mitochondria / metabolism*
  • NADPH-Ferrihemoprotein Reductase / metabolism*
  • Rats
  • Rats, Inbred Strains
  • Reference Values


  • Androstenediols
  • Isoenzymes
  • Adrenodoxin
  • Methandriol
  • Cytochrome P-450 Enzyme System
  • NADPH-Ferrihemoprotein Reductase