MiR-101 affects proliferation and apoptosis of cervical cancer cells by inhibition of JAK2

Eur Rev Med Pharmacol Sci. 2019 Jul;23(13):5640-5647. doi: 10.26355/eurrev_201907_18299.

Abstract

Objective: JAK2 expression and dysfunction play a role in tumor pathogenesis. Bioinformatics analysis revealed a targeted binding site between miR-101 and the 3'-UTR of JAK2 mRNA. This study investigated the role of miR-101 in regulating JAK2 expression and affecting the proliferation and apoptosis of cervical cancer cells.

Patients and methods: The tumor tissues and adjacent tissues of patients with cervical cancer were collected. The expression of miR-101 and JAK2 was detected by qRT-PCR. The dual luciferase reporter gene assay validated the targeting relationship between miR-101 and JAK2. The cervical cancer Caski cells were cultured in vitro, and divided into miR-NC group and miR-101 mimic group. The expression of JAK2 and p-JAK2 was detected by Western blot, cell apoptosis was detected by flow cytometry, and cell proliferation was detected by EdU staining.

Results: Compared with adjacent tissues, miR-101 expression was significantly decreased, and JAK2 expression was increased in cervical cancer tissues. There was a targeted regulatory relationship between miR-101 and JAK2. Compared with HcerEpic cells, miR-101 expression in HeLa and Caski was significantly decreased, and the expression of JAK2 and p-JAK2 was significantly increased. Transfection of miR-101 mimic significantly reduced the expression of JAK2 and p-JAK2 in Caski cells, reduced cell proliferation and increased cell apoptosis.

Conclusions: The decrease of miR-101 expression and the increase of JAK2 expression play a role in cervical cancer, while the increase of miR-101 expression can inhibit the proliferation and promote the apoptosis of cells by inhibiting the expression of JAK2.

Publication types

  • Retracted Publication

MeSH terms

  • Antagomirs / metabolism
  • Apoptosis*
  • Base Sequence
  • Cell Line, Tumor
  • Cell Proliferation*
  • Down-Regulation
  • Female
  • Humans
  • Janus Kinase 2 / antagonists & inhibitors
  • Janus Kinase 2 / genetics
  • Janus Kinase 2 / metabolism*
  • MicroRNAs / antagonists & inhibitors
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • Sequence Alignment
  • Up-Regulation
  • Uterine Cervical Neoplasms / genetics
  • Uterine Cervical Neoplasms / metabolism
  • Uterine Cervical Neoplasms / pathology*

Substances

  • Antagomirs
  • MIRN101 microRNA, human
  • MicroRNAs
  • JAK2 protein, human
  • Janus Kinase 2