Objective: The aim of this study was to investigate the potential effects of microRNA-93-5p (miR-93-5p) on the development of cervical cancer (CC), and to explore the underlying mechanism.
Patients and methods: The expression level of miR-93-5p in CC tissues and cells was detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Online prediction software and Luciferase reporter assay were used to evaluate the possible target of miR-93-5p. Furthermore, the effects of miR-93-5p on siHa cells were determined by Western blot, Cell Counting Kit-8 (CCK-8), scratch-wound and transwell assays, respectively.
Results: In our study, miR-93-5p was found highly expressed in CC tissues and cells. Thrombospondin-2 (THBS2) was predicted and experimentally verified as a direct target of miR-93-5p. Subsequent experiments showed that decreased expression of THBS2 resulting from miR-93-5p up-regulation could significantly promote the proliferation, invasion and migration of siHa cells. At the same time, miR-93-5p remarkably increased the expression of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9), whereas decreased the expression of extracellular matrix (ECM).
Conclusions: Our research discovered the promotion function of miR-93-5p on CC by targeting THBS2/Matrix metalloproteinases (MMPS) signaling pathway. We revealed that miR-93-5p might be a potential therapeutic target for the treatment of CC.