MiR-93-5p promotes cervical cancer progression by targeting THBS2/MMPS signal pathway

Eur Rev Med Pharmacol Sci. 2019 Jun;23(12):5113-5121. doi: 10.26355/eurrev_201906_18175.

Abstract

Objective: The aim of this study was to investigate the potential effects of microRNA-93-5p (miR-93-5p) on the development of cervical cancer (CC), and to explore the underlying mechanism.

Patients and methods: The expression level of miR-93-5p in CC tissues and cells was detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Online prediction software and Luciferase reporter assay were used to evaluate the possible target of miR-93-5p. Furthermore, the effects of miR-93-5p on siHa cells were determined by Western blot, Cell Counting Kit-8 (CCK-8), scratch-wound and transwell assays, respectively.

Results: In our study, miR-93-5p was found highly expressed in CC tissues and cells. Thrombospondin-2 (THBS2) was predicted and experimentally verified as a direct target of miR-93-5p. Subsequent experiments showed that decreased expression of THBS2 resulting from miR-93-5p up-regulation could significantly promote the proliferation, invasion and migration of siHa cells. At the same time, miR-93-5p remarkably increased the expression of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9), whereas decreased the expression of extracellular matrix (ECM).

Conclusions: Our research discovered the promotion function of miR-93-5p on CC by targeting THBS2/Matrix metalloproteinases (MMPS) signaling pathway. We revealed that miR-93-5p might be a potential therapeutic target for the treatment of CC.

MeSH terms

  • 3' Untranslated Regions
  • Cell Line, Tumor
  • Cell Movement
  • Cell Proliferation
  • Disease Progression
  • Female
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Matrix Metalloproteinases / genetics
  • MicroRNAs / genetics*
  • Thrombospondins / genetics*
  • Thrombospondins / metabolism
  • Up-Regulation*
  • Uterine Cervical Neoplasms / genetics*
  • Uterine Cervical Neoplasms / metabolism

Substances

  • 3' Untranslated Regions
  • MIRN93 microRNA, human
  • MicroRNAs
  • Thrombospondins
  • thrombospondin 2
  • Matrix Metalloproteinases