Pyrazinamidase activity of Mycobacterium tuberculosis--a test of sensitivity to pyrazinamide

Tubercle. 1987 Sep;68(3):221-4. doi: 10.1016/0041-3879(87)90058-4.


Pyrazinamidase activity has been found to correlate with pyrazinamide sensitivity in strains of Mycobacterium tuberculosis. In vitro sensitivity to pyrazinamide in acidified Löwenstein-Jensen medium, and pyrazinamidase activity by the Wayne method, were determined in 378 clinical isolates of M. tuberculosis. A close correlation was observed between the results of both tests. This method of detecting pyrazinamidase activity was found to be a rapid, simple and reliable substitute for pyrazinamide sensitivity testing, and it overcomes the difficulty of growing M. tuberculosis at pH 5.5, as required in the standard method.

MeSH terms

  • Amidohydrolases / metabolism*
  • Microbial Sensitivity Tests
  • Mycobacterium tuberculosis / drug effects
  • Mycobacterium tuberculosis / enzymology*
  • Pyrazinamide / pharmacology*


  • Pyrazinamide
  • Amidohydrolases
  • pyrazinamide deamidase