Definition of Liquid and Powder Cellulase Formulations Using Domestic Wastewater in Bubble Column Reactor

Appl Biochem Biotechnol. 2020 Jan;190(1):113-128. doi: 10.1007/s12010-019-03075-1. Epub 2019 Jul 13.


Raw domestic wastewater was used as a culture medium for cellulase production in a bubble column reactor (6.2 UFP/mL, 64.6 U/L h) using the strain Trichoderma harzianum TRIC03-LPBII. Cellulases presented optimum pH and temperature between 4 and 5 and 50 and 70 °C, respectively. Enzymatic extract was concentrated through ultrafiltration and then a cellulolytic formulation was prepared with the addition of sorbitol (50% w/v) and benzoic acid (0.05% w/v). High cellulase stability of around 100% was reached after 30 days at 4 °C. The concentrated extract was also dried in a spray-dryer with the addition of maltodextrin at 20% (w/v), resulting in powder enzymatic formulation with 85% stability after 60 days. With these characteristics, the liquid and powder cellulase products have potential to be used in different industrial applications.

Keywords: Bubble column bioreactor; Domestic wastewater; Enzyme formulation; FPase; Trichoderma harzianum; β-Glucosidases.

MeSH terms

  • Bioreactors*
  • Cellulase / metabolism*
  • Culture Media
  • Enzyme Stability
  • Fermentation
  • Hydrogen-Ion Concentration
  • Kinetics
  • Powders*
  • Temperature
  • Trichoderma / enzymology
  • Trichoderma / metabolism
  • Wastewater / chemistry*


  • Culture Media
  • Powders
  • Waste Water
  • Cellulase