Evaluation of Kidney Function Parameters in Diabetic Rats Following Virgin Coconut Oil Diet

Folia Med (Plovdiv). 2019 Jun 1;61(2):249-257. doi: 10.2478/folmed-2018-0083.

Abstract

Background: Diabetes mellitus (DM) leads to disruption of kidney function parameters (KFPs) which are markers of kidney diseases, especially nephropathy. Virgin coconut oil (VCO) has been implicated in playing a significant role in DM management. However, its role on KFPs in DM is scarce.

Aim: To evaluate the kidney function parameters following VCO diet in diabetic rats.

Materials and methods: Twenty-five (25) male rats of 150 - 200 g were divided into 5 groups (n=5): Non-diabetic control (Group 1), diabetes control (Group 2), diabetes + metformin (Group 3), diabetes + 10% VCO (Group 4) and diabetes + 20% VCO (Group 5). Apart from Group 1, other groups were given intraperitone-ally 50 mg/kg of streptozotocin to induce diabetes mellitus. After 72 hours, fasting hyperglycaemia was confirmed by glucose oxidase method. All the rats were fed normal rat chow for 8 weeks. At 8th week, serum and urine samples were analysed for biochemical analysis. After 8 weeks, Group 1 and Group 2 continued to be fed on normal rat chow while other groups were treated with diets (VCO) or drug (metformin) for 4 weeks. At 12th week, urine samples were collected for biochemical analysis, the rats were sacrificed, and blood samples were collected by cardiac puncture.

Results: There were significant differences in some KFPs in diabetes control (Group 2) compared to other experimental groups. However, there was no significant difference in glomerular filtration rate (GFR) and serum sodium in all the groups.

Conclusion: VCO supplementary diet improved the altered KFPs and could be a therapy for kidney problems.

Keywords: diabetes mellitus; diabetic rat; diet; kidney function parameters; virgin coconut oil.

MeSH terms

  • Animals
  • Blood Urea Nitrogen
  • Body Weight
  • Coconut Oil / pharmacology*
  • Creatinine / metabolism
  • Diabetes Mellitus, Experimental / metabolism*
  • Diabetic Nephropathies / metabolism*
  • Diet
  • Glomerular Filtration Rate / drug effects*
  • Hypoglycemic Agents / pharmacology
  • Kidney / drug effects*
  • Kidney / metabolism
  • Kidney / pathology
  • Male
  • Metformin / pharmacology
  • Organ Size / drug effects
  • Rats
  • Serum Albumin / drug effects

Substances

  • Hypoglycemic Agents
  • Serum Albumin
  • Metformin
  • Creatinine
  • Coconut Oil