A 'catch-and-release' receptor for the cholera toxin

Faraday Discuss. 2019 Oct 30;219(0):112-127. doi: 10.1039/c9fd00017h.


Stimuli-responsive receptors for the recognition unit of the cholera toxin (CTB) have been prepared by attaching multiple copies of its natural carbohydrate ligand, the GM1 oligosaccharide, to a thermoresponsive polymer scaffold. Below their lower critical solution temperature (LCST), polymers complex CTB with nanomolar affinity. When heated above their LCST, polymers undergo a reversible coil to globule transition which renders a proportion of the carbohydrate recognition motifs inaccessible to CTB. This thermally-modulated decrease in the avidity of the material for the protein has been used to reversibly capture CTB from solution, enabling its convenient isolation from a complex mixture.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cholera / microbiology
  • Cholera Toxin / metabolism*
  • G(M1) Ganglioside / chemistry
  • G(M1) Ganglioside / metabolism*
  • Humans
  • Organoids
  • Phase Transition
  • Polymers / chemistry
  • Polymers / metabolism*
  • Protein Binding
  • Temperature
  • Vibrio cholerae / enzymology*


  • Polymers
  • G(M1) Ganglioside
  • Cholera Toxin