Reverse ELISPOT assay for clonal analysis of cytokine production. I. Enumeration of gamma-interferon-secreting cells

J Immunol Methods. 1988 May 25;110(1):29-36. doi: 10.1016/0022-1759(88)90079-8.


A reverse modification of ELISPOT assay using nitrocellulose membranes and epitope-specific monoclonal antibodies is described for the detection of single lymphokine-secreting cells. As a model, the production of gamma-interferon by mitogen stimulated human peripheral blood lymphocytes has been examined. The assay can also be modified to permit microscopic examination of spot-forming cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / immunology
  • Collodion
  • Cycloheximide / pharmacology
  • Enzyme-Linked Immunosorbent Assay / instrumentation
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Humans
  • Interferon-gamma / immunology
  • Interferon-gamma / metabolism*
  • Leukocytes, Mononuclear / metabolism*
  • Membranes, Artificial


  • Antibodies, Monoclonal
  • Membranes, Artificial
  • Interferon-gamma
  • Collodion
  • Cycloheximide