Abstract
A reverse modification of ELISPOT assay using nitrocellulose membranes and epitope-specific monoclonal antibodies is described for the detection of single lymphokine-secreting cells. As a model, the production of gamma-interferon by mitogen stimulated human peripheral blood lymphocytes has been examined. The assay can also be modified to permit microscopic examination of spot-forming cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Monoclonal / immunology
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Collodion
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Cycloheximide / pharmacology
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Enzyme-Linked Immunosorbent Assay / instrumentation
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Enzyme-Linked Immunosorbent Assay / methods*
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Humans
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Interferon-gamma / immunology
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Interferon-gamma / metabolism*
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Leukocytes, Mononuclear / metabolism*
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Membranes, Artificial
Substances
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Antibodies, Monoclonal
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Membranes, Artificial
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Interferon-gamma
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Collodion
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Cycloheximide