Establishment of an immature mast cell line from a patient with mast cell leukemia

Leuk Res. 1988;12(4):345-55. doi: 10.1016/0145-2126(88)90050-1.


A cell line showing many characteristics of immature mast cells has been established from the peripheral blood of a patient with mast cell leukemia. Cultured cells contain low levels of histamine, are stained metachromatically by toluidine blue, and contain chloroacetate esterase, aminocaproate esterase and tryptase activities. The cells lack T and B lymphocyte, as well as myeloid cell markers, and do not possess IgE receptors. Solid tumors of metachromatically positive cells have been successfully induced and serially passed in nude mice using 5-azacytidine transformed cells. This cell line may be useful for future studies of mast cells and their constituents.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens, Surface / analysis
  • Cell Line*
  • Clone Cells
  • Humans
  • Leukemia, Mast-Cell / genetics
  • Leukemia, Mast-Cell / pathology*
  • Mast Cells*
  • Mice
  • Mice, Nude
  • Middle Aged
  • Neoplasm Transplantation
  • Translocation, Genetic


  • Antigens, Surface