Measuring vision in rodents is a critical step for understanding vision, improving models of human disease, and developing therapies. Established behavioural tests for perceptual vision, such as the visual water task, rely on learning. The learning process, while effective for sighted animals, can be laborious and stressful in animals with impaired vision, requiring long periods of training. Current tests that that do not require training are based on sub-conscious, reflex responses (e.g. optokinetic nystagmus) that don't require involvement of visual cortex and higher order thalamic nuclei. A potential alternative for measuring vision relies on using visually guided innate defensive responses, such as escape or freeze, that involve cortical and thalamic circuits. In this study we address this possibility in mice with intact and degenerate retinas. We first develop automatic methods to detect behavioural responses based on high dimensional tracking and changepoint detection of behavioural time series. Using those methods, we show that visually guided innate responses can be elicited using parametisable stimuli, and applied to describing the limits of visual acuity in healthy animals and discriminating degrees of visual dysfunction in mouse models of retinal degeneration.
Conflict of interest statement
The authors declare no competing interests.
Psychophysical testing in rodent models of glaucomatous optic neuropathy.Exp Eye Res. 2015 Dec;141:154-63. doi: 10.1016/j.exer.2015.06.025. Epub 2015 Jul 2. Exp Eye Res. 2015. PMID: 26144667 Free PMC article. Review.
A Mouse Model of Visual Perceptual Learning Reveals Alterations in Neuronal Coding and Dendritic Spine Density in the Visual Cortex.Front Behav Neurosci. 2016 Mar 10;10:42. doi: 10.3389/fnbeh.2016.00042. eCollection 2016. Front Behav Neurosci. 2016. PMID: 27014004 Free PMC article.
Visual input to the mouse lateral posterior and posterior thalamic nuclei: photoreceptive origins and retinotopic order.J Physiol. 2016 Apr 1;594(7):1911-29. doi: 10.1113/JP271707. J Physiol. 2016. PMID: 26842995 Free PMC article.
Detailed Visual Cortical Responses Generated by Retinal Sheet Transplants in Rats with Severe Retinal Degeneration.J Neurosci. 2018 Dec 12;38(50):10709-10724. doi: 10.1523/JNEUROSCI.1279-18.2018. Epub 2018 Nov 5. J Neurosci. 2018. PMID: 30396913 Free PMC article.
Tests of the mouse visual system.Mamm Genome. 2000 Jul;11(7):531-6. doi: 10.1007/s003350010102. Mamm Genome. 2000. PMID: 10886018 Review.
- MR/N012992/1/MRC_/Medical Research Council/United Kingdom
- NC/P001505/1/National Centre for the Replacement, Refinement and Reduction of Animals in Research/United Kingdom
- NC/P001505/1/National Centre for the Replacement Refinement and Reduction of Animals in Research (NC3Rs)
- MR/N012992/1/RCUK | Medical Research Council (MRC)