Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Jul 3:19:100663.
doi: 10.1016/j.bbrep.2019.100663. eCollection 2019 Sep.

Screening and selection of bacteria inhibiting white spot syndrome virus infection to Litopenaeus vannamei

Ashokkumar Sekar  1 , Myungjin Kim  1 , Hantaek Jeon  2 , Keun Kim  1
Affiliations

Screening and selection of bacteria inhibiting white spot syndrome virus infection to Litopenaeus vannamei

Ashokkumar Sekar et al. Biochem Biophys Rep. .

Abstract

A total of 173 bacterial strains were isolated from different sources at different regions such as fermented foods, shrimp guts, sea water, mangrove water, and sediments. These bacteria were screened against white spot syndrome virus (WSSV) infection in Palaemon paucidens. Based on mortality, white spot level, and healthiness, three bacterial strains were selected and identified using 16S rRNA gene sequencing. These bacterial strains were Bacillus subtilis KA1, B. licheniformis KA2, and B. subtilis KA3. WSSV challenge test in pilot scale was conducted using Litopenaeus vannamei with B. subtilis KA1 and B. subtilis KA3. The survival ratio of shrimp was 0% for WSSV control after 17th days, 84% for B. subtilis KA1 plus WSSV after 26th days, and 28% for B. subtilis KA3 with WSSV after 26th days. B. subtilis KA1 showed good growth at 18-37 °C in with and without 3% NaCl, and therefore can be applied to aquaculture at low to high temperatures. B. subtilis KA1 produced protease and lipase which can increase digestion to shrimp; exhibited antibacterial activity against Vibrio parahaemolyticus; and significantly increased the survival of WSSV challenged shrimps.

Keywords: Bacillus subtilis; Probiotics; Schrimp; Vibrio parahaemolyticus; White spot syndrome virus.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Survival ratio of WSSV infected L. vannamei.
Fig. 2
Fig. 2
WSSV infection: Animal control (A), WSSV control (B), and low infection with Bacillus subtilis KA1 (C).
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Chou H.Y., Huang C.Y., Lo C.F., Kou G.H. Studies on transmission of white spot syndrome associated baculovirus (WSBV) in Penaeus monodon and P. japonicus via waterborne contact and oral ingestion. Aquaculture. 1998;164:263–276.
    1. Van Thuong K., Van Tuan V., Li W., Sorgeloos P., Bossier P., Nauwynck H. Effects of acute change in salinity and moulting on the infection of white leg shrimp (Penaeus vannamei) with white spot syndrome virus upon immersion challenge. J. Fish Dis. 2016;39(12):1403–1412. - PubMed
    1. He W., Rahimnejad S., Wang L., Song K., Lu K., Zhang C. Effects of organic acids and essential oils blend on growth, gut microbiota, immune response and disease resistance of Pacific white shrimp (Litopenaeus vannamei) against Vibrio parahaemolyticus. Fish Shellfish Immunol. 2017;70:164–173. - PubMed
    1. Suo Y., Li E., Li T., Jia Y., Qin J.G., Gu Z., Chen L. Response of gut health and microbiota to sulfide exposure in Pacific white shrimp Litopenaeus vannamei. Fish Shellfish Immunol. 2017;63:87–96. - PubMed
    1. Nakano H., Hiraoka M., Sameshima M., Kimura T., Momoyama K. Inactivation of penaeid rod-shaped DNA virus (PRDV), the causative agent of Penaied Acute Viremia (PAV), by some chemical and physical treatments. Fish Pathol. 1998;33:65–72.

LinkOut - more resources