Distribution of type I, II, III and V in the pepsin solubilized collagens in bovine menisci

Connect Tissue Res. 1987;16(4):343-56. doi: 10.3109/03008208709005619.


The inner one-third (IM) of both lateral and medial menisci resembles hyaline cartilage, both in gross appearance and histological examination, while the outer two-thirds (OM) is fibrocartilaginous in appearance. Collagen was extracted with pepsin, purified with anion and cation exchange column chromatographies and examined by differential salt precipitation, cyanogen bromide-peptide analysis and SDS gel electrophoresis. IM constitutes approximately 10% of the wet weight of whole meniscus, is made up of 70% collagen of which 34% is pepsin soluble. IM is composed of 60% type II and 40% type I collagen. OM is made up of 80% collagen of which 17% is pepsin soluble. The predominant collagen of OM is type I with a trace amount of types III and V (less than 1%).

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cartilage, Articular / analysis
  • Cattle
  • Chemical Precipitation
  • Chromatography, Ion Exchange
  • Collagen / analysis*
  • Cyanogen Bromide
  • Electrophoresis, Polyacrylamide Gel
  • Menisci, Tibial / analysis*
  • Pepsin A*
  • Skin / analysis
  • Solubility


  • Collagen
  • Pepsin A
  • Cyanogen Bromide