Characterization of 3 PET Tracers for Quantification of Mitochondrial and Synaptic Function in Healthy Human Brain: 18 F-BCPP-EF, 11 C-SA-4503, and 11 C-UCB-J

J Nucl Med. 2020 Jan;61(1):96-103. doi: 10.2967/jnumed.119.228080. Epub 2019 Jul 19.

Abstract

Mitochondrial complex 1 is involved in maintaining brain bioenergetics; σ-1 receptor responds to neuronal stress; and synaptic vesicle protein 2A reflects synaptic integrity. Expression of each of these proteins is altered in neurodegenerative diseases. Here, we characterize the kinetic behavior of 3 PET radioligands-18F-BCPP-EF, 11C-SA-4503, and 11C-UCB-J-for the measurement of mitochondrial complex 1, σ-1 receptor, and synaptic vesicle protein 2A, respectively, and determine appropriate analysis workflows for their application in future studies of the in vivo molecular pathology of these diseases. Methods: Twelve human subjects underwent dynamic PET scans with each radioligand, including associated arterial blood sampling. A range of kinetic models was investigated to identify an optimal kinetic analysis method for each radioligand and a suitable acquisition duration. Results: All 3 radioligands readily entered the brain and yielded heterogeneous uptake consistent with the known distribution of the targets. The optimal models determined for the regional estimates of volume of distribution were multilinear analysis 1 (MA1) and the 2-tissue-compartment model for 18F-BCPP-EF, MA1 for 11C-SA-4503, and both MA1 and the 1-tissue-compartment model for 11C-UCB-J. Acquisition times of 70, 80, and 60 min for 18F-BCPP-EF, 11C-SA-4503, 11C-UCB-J, respectively, provided good estimates of regional volume of distribution values. An effect of age was observed on 18F-BCPP-EF and 11C-UCB-J signal in the caudate. Conclusion: These ligands can be assessed for their potential to stratify patients or monitor the progression of molecular neuropathology in neurodegenerative diseases.

Keywords: endoplasmic reticulum; kinetic modeling; mitochondria; neurodegeneration; synapses.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Age Factors
  • Aged
  • Aging
  • Brain / diagnostic imaging*
  • Endoplasmic Reticulum / metabolism
  • Female
  • Humans
  • Image Processing, Computer-Assisted
  • Kinetics
  • Ligands
  • Male
  • Middle Aged
  • Mitochondria / metabolism
  • Neurodegenerative Diseases / diagnostic imaging
  • Piperazines / chemistry*
  • Positron-Emission Tomography*
  • Pyridazines / chemistry*
  • Pyridines / chemistry*
  • Pyrrolidinones / chemistry*
  • Radiopharmaceuticals / chemistry*
  • Time Factors

Substances

  • 1-((3-(methylpyridin-4-yl)methyl)-4-(3,4,5-trifluorophenyl)pyrrolidin-2-one
  • 2-tert-butyl-4-chloro-5-(6-(2-(2-fluoroethoxy)ethoxy)pyridin-3-ylmethoxy)-2H-pyridazin-3-one
  • Ligands
  • Piperazines
  • Pyridazines
  • Pyridines
  • Pyrrolidinones
  • Radiopharmaceuticals
  • SA 4503