Effects of Amanita muscaria extract on different in vitro neurotoxicity models at sub-cellular and cellular levels

Food Chem Toxicol. 2019 Oct:132:110687. doi: 10.1016/j.fct.2019.110687. Epub 2019 Jul 17.


Muscimol is the main compound found in Amanita muscaria. Several studies have proven that muscimol has suppressive effects on essential tremor, without impairing speech and coordination. The effects of muscimol in Parkinson-affected patients is also described in a number of studies. These studies describe the free radical scavenging and antioxidant activity of the mushroom extract. We have evaluated the possible neuroprotective effects of a standardized extract from A. muscaria, containing high amounts of muscimol, on different models of neurotoxicity in rat brain microsomes, mitochondria, synaptosomes as well as on neuroblastoma cell line SH-SY5Y. The possible inhibitory effect on human recombinant monoaminoxidase-B (hMAOB) enzyme was also studied. The extract revealed statistically significant neuroprotective effects on the in vitro neurotoxicity models and no inhibitory activity on hMAOB.

Keywords: Amanita muscaria; Human recombinant monoaminoxidase-B enzyme; Isolated rat brain microsomes; Mitochondria; Neuroblastoma cell line SH-SY5Y; Neuroprotection; Synaptosomes.

MeSH terms

  • Amanita / chemistry*
  • Animals
  • Antioxidants / pharmacology
  • Brain / drug effects
  • Cell Line, Tumor
  • Humans
  • Male
  • Monoamine Oxidase / drug effects
  • Muscimol / isolation & purification
  • Muscimol / pharmacology*
  • Neuroprotective Agents / pharmacology
  • Organelles / drug effects
  • Rats
  • Rats, Wistar


  • Antioxidants
  • Neuroprotective Agents
  • Muscimol
  • Monoamine Oxidase