Effect of chemotactic factors on hexose transport in polymorphonuclear leucocytes

Biochim Biophys Acta. 1988 Jun 22;941(2):157-64. doi: 10.1016/0005-2736(88)90176-9.


Transport of the nonmetabolizable glucose analogue, 3-O-methylglucose, was assessed in human polymorphonuclear leucocytes with or without the chemotactic peptide N-formylmethionylleucylphenylalanine (fMet-Leu-Phe). The peptide increased entry of labelled 3-O-methylglucose about 5-fold and the intracellular distribution space about 70%. The half-time of equilibration was 3 s in the treated cells. Similar effects were observed with zymosan-treated serum (containing the chemotactic factor C5a), with arachidonic acid, calcium ionophore A23187 and phorbol myristate acetate. However, the chemotactic protein, thrombin, had no effect, even though binding to high-affinity receptors was demonstrated. Km for zero-trans entry of 3-O-methylglucose was about 1 mM and fMet-Leu-Phe increased Vmax from 5 to about 25 amol.s-1.cell-1. Similar values were obtained from incubations for a few seconds with glucose and 2-deoxyglucose. The rate of 2-deoxyglucose uptake (8 min incubations) was limited by the transport step at substrate concentrations lower than approx. 0.1 mM, whereas the phosphorylation step became rate-limiting at higher concentrations. Thus, 2-deoxyglucose uptake can only be taken as a measure of transport at a tracer concentration. It is concluded that chemotactic factors can, but do not necessarily, increase the maximal transport velocity of hexoses entering the polymorphonuclear leucocyte via the glucose transporter.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3-O-Methylglucose
  • Arachidonic Acid
  • Arachidonic Acids / pharmacology
  • Biological Transport / drug effects
  • Blood
  • Calcimycin / pharmacology
  • Chemotactic Factors / pharmacology*
  • Deoxyglucose / blood
  • Glucose / metabolism
  • Hexoses / blood*
  • Humans
  • Kinetics
  • Methylglucosides / blood
  • Monosaccharide Transport Proteins / blood
  • N-Formylmethionine Leucyl-Phenylalanine / pharmacology
  • Neutrophils / metabolism*
  • Tetradecanoylphorbol Acetate / pharmacology
  • Thrombin / pharmacology
  • Zymosan / pharmacology


  • Arachidonic Acids
  • Chemotactic Factors
  • Hexoses
  • Methylglucosides
  • Monosaccharide Transport Proteins
  • 3-O-Methylglucose
  • Arachidonic Acid
  • Calcimycin
  • N-Formylmethionine Leucyl-Phenylalanine
  • Zymosan
  • Deoxyglucose
  • Thrombin
  • Glucose
  • Tetradecanoylphorbol Acetate