TAF-ChIP: an ultra-low input approach for genome-wide chromatin immunoprecipitation assay

Life Sci Alliance. 2019 Jul 22;2(4):e201900318. doi: 10.26508/lsa.201900318. Print 2019 Aug.


Chromatin immunoprecipitation (ChIP) followed by next generation sequencing (ChIP-Seq) is a powerful technique to study transcriptional regulation. However, the requirement of millions of cells to generate results with high signal-to-noise ratio precludes it in the study of small cell populations. Here, we present a tagmentation-assisted fragmentation ChIP (TAF-ChIP) and sequencing method to generate high-quality histone profiles from low cell numbers. The data obtained from the TAF-ChIP approach are amenable to standard tools for ChIP-Seq analysis, owing to its high signal-to-noise ratio. The epigenetic profiles from TAF-ChIP approach showed high agreement with conventional ChIP-Seq datasets, thereby underlining the utility of this approach.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chromatin Immunoprecipitation Sequencing / methods*
  • Drosophila / genetics*
  • Epigenesis, Genetic
  • High-Throughput Nucleotide Sequencing
  • Histones / metabolism*
  • Humans
  • K562 Cells
  • Signal-To-Noise Ratio
  • Software
  • Whole Genome Sequencing


  • Histones