Reversible arrest of proliferation of rat 3Y1 fibroblasts in both the G1 and G2 phases by trichostatin A

Exp Cell Res. 1988 Jul;177(1):122-31. doi: 10.1016/0014-4827(88)90030-4.


Proliferation of rat 3Y1 cells was found to be specifically blocked by trichostatin A (TSA) at two distinct stages in the cell cycle. The first block occurred in the early G1 phase at least 9 h before the G1/S boundary, whereas the second occurred during the G2 phase. When TSA-arrested cells at the G2 phase were released from the inhibition, the cells with 4C DNA entered a new S phase without passage through the M phase, resulting in the formation of proliferative tetraploid cells. The removal of TSA induced a rapid transient increase in the transcription of c-fos and the cells required 15 h to enter the S phase after release. These results suggest that the cells arrested with TSA are quiescent (G0).

MeSH terms

  • Animals
  • Cell Division / drug effects
  • Cell Line
  • Clone Cells
  • DNA / biosynthesis
  • Embryo, Mammalian
  • Fibroblasts / cytology*
  • Gene Expression Regulation / drug effects
  • Hydroxamic Acids / pharmacology
  • Interphase / drug effects*
  • Kinetics
  • Mitosis / drug effects
  • Polyploidy
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins c-fos
  • Rats


  • Hydroxamic Acids
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-fos
  • trichostatin A
  • DNA