Detecting amplicons of loop-mediated isothermal amplification

Microbiol Immunol. 2019 Oct;63(10):407-412. doi: 10.1111/1348-0421.12734. Epub 2019 Aug 13.

Abstract

Loop-mediated isothermal amplification (LAMP) assays are used to detect diverse pathogens. Initially, LAMP amplicons were detected using electrophoresis; later, real-time monitoring based on turbidity was developed to overcome the problem of contamination with environmental DNA. Recently, real-time monitoring of fluorescence signals using a quenching primer and probe has improved the reliability of amplification signals. Here, methods of detecting LAMP amplicons are reviewed.

Keywords: LAMP; QPrimer; QProbe; detection; turbidity.

Publication types

  • Review

MeSH terms

  • Bacterial Infections / microbiology*
  • DNA, Bacterial / analysis*
  • DNA, Protozoan / analysis*
  • DNA, Viral / analysis*
  • Fluorescence
  • Nephelometry and Turbidimetry / methods
  • Nucleic Acid Amplification Techniques / methods*
  • Protozoan Infections / parasitology*
  • Reproducibility of Results
  • Virus Diseases / virology*

Substances

  • DNA, Bacterial
  • DNA, Protozoan
  • DNA, Viral