Binding sites for 125I-cholecystokinin in primate spinal cord are of the CCK-A subclass

Neurosci Lett. 1988 Jun 29;89(2):133-9. doi: 10.1016/0304-3940(88)90369-2.


Cholecystokinin (CCK) receptor binding was measured in sections of human, monkey and rat spinal cord using autoradiographical techniques. In each species, high levels of specific 125I-Bolton-Hunter CCK binding were detected in the superficial layers of the dorsal horn (the substantia gelatinosa). In monkey and human but not rat spinal cord, 125I-CCK binding was dose-dependently inhibited by low concentrations of the selective CCK-A antagonist L-364,718. Binding of [3H]L-364,718, which was saturable (Bmax = 29.0 +/- 0.95 pmol/g wet wt.) and of high affinity (pKd) = 9.92 +/- 0.16) was also detected in sections of monkey spinal cord and had a similar localization to that of specific 125I-CCK binding. These data indicate that in striking contrast to CCK receptors in rat spinal cord, those in the primate cord are of the CCK-A receptor subclass.

MeSH terms

  • Animals
  • Benzodiazepinones / metabolism
  • Cholecystokinin / classification
  • Cholecystokinin / metabolism*
  • Devazepide
  • Haplorhini
  • Humans
  • Iodine Radioisotopes
  • Rats
  • Receptors, Cholecystokinin / metabolism*
  • Spinal Cord / metabolism*


  • Benzodiazepinones
  • Iodine Radioisotopes
  • Receptors, Cholecystokinin
  • Cholecystokinin
  • Devazepide