Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 11 (6), e547-e552

Comparative Evaluation of Sealing Ability, Penetration and Adaptation of a Self Etching Pit and Fissure Sealant- Stereomicroscopic and Scanning Electron Microscopic Analyses


Comparative Evaluation of Sealing Ability, Penetration and Adaptation of a Self Etching Pit and Fissure Sealant- Stereomicroscopic and Scanning Electron Microscopic Analyses

Dhruv Garg et al. J Clin Exp Dent.


Background: The efficacy of pit and fissure sealants in preventing occlusal caries is a well-established fact. Considering the difficulty in achieving strict isolation for a longer duration while treating the pediatric patients, a simplified procedure of sealant application is desirable. While, a self-etching sealant, Prevent Seal offers a quick procedure, the physical properties of this material haven't been studied yet. Thus, this study was aimed to comparatively evaluate sealing ability, penetration and adaptation of a self-etching pit and fissure sealant and a conventional resin sealant.

Material and methods: This was an in vitrointergroup comparative study, which consisted of 2 groups- Group I (Conventional acid etch sealant, Clinpro) and Group II (Self etching sealant, Prevent Seal). Out of 32 selected teeth 16 were used to study microleakage, with the help of dye penetration test using Övrebö and Raadal criteria. Remaining 16 were used to evaluate sealant penetration and adaptation viz bubbles in the bottom of fissure, debris in the fissure, tags in the bottom of the fissure and tags at cuspal slopes and fissure entrance was done using stereomicroscope. Post stereomicroscopic evaluation 4 samples each were randomly chosen from both the groups and checked for etching pattern using Scanning electronic microscope.

Results: The comparison of tested properties between the groups was done using Chi square test. There was no statistically significant difference observed when microleakage and sealant penetration / adaptation properties were compared between two groups (p=0.63 and p= 0.131, 0.131, 0.302, 0.106 respectively). No conclusive results could be withdrawn while etching patterns were compared between the groups (p=0.717).

Conclusions: The self-etching sealant Prevent seal was found to have similar microleakage, sealant penetration and adaptation properties as conventional acid etch sealant. Key words:Pit and fissure sealant, self-etching, scanning electron microscope, sealing ability.

Conflict of interest statement

Conflict of interest statement:The authors have declared that no conflict of interest exist.


Figure 1
Figure 1
Figure 2
Figure 2
Different microleakage scores as seen by stereomicroscopic examination: A) Score 0, B) Score 1, C) Score 2, D) Score 3.
Figure 3
Figure 3
A) Bubbles and debris visible on examining with stereomicroscope and B) Tags present on the bottom and slopes of the fissure.
Figure 4
Figure 4
A-C) Etching patterns seen in Group I under Scanning Electron Microscope- A) Type I (magnification X 1.00K), B) Type II (magnification X 1.00K), C) Type III (magnification X 600K), D-F) Etching patterns seen in Group II under Scanning Electron Microscope- D) Type I (magnification X 1.00K), E) Type II (magnification X 600K), F) Type III (magnification X 600K).

Similar articles

See all similar articles


    1. Wright JT, Tampi MP, Graham L, Estrich C, Crall JJ, Fontana M. Sealants for Preventing and Arresting Pit-and-fissure Occlusal Caries in Primary and Permanent Molars. Pediatr Dent. 2016;38:282–308. - PubMed
    1. Weerheijm KL. Molar incisor hypomineralisation (MIH) Eur J Paediatr Dent. 2003;4:114–20. - PubMed
    1. Kotsanos N, Kaklamanos EG, Arapostathis K. Treatment management of first permanent molars in children with Molar Incisor Hypomineralisation. Eur J Paediatr Dent. 2005;6:179–84. - PubMed
    1. Welbury R, Raadal MLN, European Academy of Paediatric Dentistry. Guidelines for the use of pit and fissure sealants. Eur J Paediatr Dent. 2004;5:179–84. - PubMed
    1. Bevilacqua L, Cadenaro M, Sossi A, Biasotto M, Di Lenarda R. Influence of air abrasion and etching on enamel and adaptation of a dental sealant. Eur J Paediatr Dent. 2007;8:25–30. - PubMed

LinkOut - more resources