Relationships between Body Composition and Plasma Levels of Pancreatic, Gut, and Adipose Tissue Hormones in db/db Mice, a Model of Type 2 Diabetes Mellitus

Bull Exp Biol Med. 2019 Jul;167(3):325-328. doi: 10.1007/s10517-019-04519-8. Epub 2019 Jul 26.


We studied the relationships between body composition parameters and plasma levels of pancreatic, gut, and adipose tissue hormones regulating energy balance and glucose metabolism in diabetic db/db mice (BKS.Cg-Dock7m+/+Leprdb/J). The body composition parameters in mice aged 8, 12, and 16 weeks were assessed by magnetic resonance imaging. The concentrations of insulin, glucagon, ghrelin, glucagon-like peptide-1, glucose-dependent immunotropic peptide, leptin, resistin, and plasminogen activator-1 were measured by multiplex analysis at the age of 8 and 16 weeks. In comparison with non-diabetic control (db/+), db/db mice demonstrated high fat mass and reduced lean body mass and water content. In 8- and 16-week-old db/db mice, the levels of leptin (p<0.001), insulin (p<0.01), and glucagon-like peptide-1 (p<0.05) were elevated and the concentration of ghrelin (p<0.05) was reduced. The body weight and fat mass positively correlated with the levels of leptin, insulin, plasminogen activator-1, and glucagon-like peptide-1 and negatively correlated with ghrelin concentration. The results provide further details for characteristics of db/db mice, a widely used model of obesity and type 2 diabetes mellitus.

Keywords: body composition; db/db mice; diabetes mellitus; hormone; obesity.

MeSH terms

  • Adipose Tissue / metabolism*
  • Animals
  • Body Composition / physiology*
  • Diabetes Mellitus, Type 2 / blood*
  • Gastrointestinal Hormones / blood*
  • Ghrelin / blood
  • Glucagon / blood
  • Glucagon-Like Peptide 1 / blood
  • Glucose / metabolism
  • Insulin / blood
  • Leptin / blood
  • Male
  • Mice
  • Mice, Inbred NOD
  • Pancreatic Hormones / blood*
  • Plasminogen Activators / blood
  • Resistin / blood


  • Gastrointestinal Hormones
  • Ghrelin
  • Insulin
  • Leptin
  • Pancreatic Hormones
  • Resistin
  • Glucagon-Like Peptide 1
  • Glucagon
  • Plasminogen Activators
  • Glucose