Counting the Cuts: MAZTER-Seq Quantifies m6A Levels Using a Methylation-Sensitive Ribonuclease

Radha Raman Pandey; Ramesh S Pillai

doi:10.1016/j.cell.2019.07.006

Counting the Cuts: MAZTER-Seq Quantifies m⁶A Levels Using a Methylation-Sensitive Ribonuclease

Cell. 2019 Jul 25;178(3):515-517. doi: 10.1016/j.cell.2019.07.006.

Authors

Radha Raman Pandey¹, Ramesh S Pillai²

Affiliations

¹ Department of Molecular Biology, Science III, University of Geneva, 30 Quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland.
² Department of Molecular Biology, Science III, University of Geneva, 30 Quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland. Electronic address: ramesh.pillai@unige.ch.

PMID: 31348883
DOI: 10.1016/j.cell.2019.07.006

Abstract

Garcia-Campos et al. describe MAZTER-seq, which deploys a sequence-specific, methylation-sensitive bacterial single-stranded ribonuclease MazF to provide nucleotide-resolution quantification of m⁶A methylation sites. The study reveals many new sites and supports the idea of a predictable "m⁶A code," where methylation levels are dictated primarily by local sequence at the site of methylation.

Publication types

Comment

MeSH terms

Base Sequence
DNA Methylation*
Ribonucleases*

Substances

Ribonucleases