Lachnoanaerobaculum gingivalis sp. nov., Isolated from Human Subgingival Dental Plaque of a Gingivitis Lesion

Abstract

A novel Gram-stain-positive, obligately anaerobic, spore-forming rod, designated strain ChDC B114^T, was isolated from a human dental plaque of a gingivitis lesion. The strain was characterized by polyphasic taxonomic analysis to identify it at the species level. The 16S ribosomal RNA gene (16S rDNA) sequence analysis revealed that the strain belongs to the genus Lachnoanaerobaculum. The percent similarity of the 16S rDNA of the strain was closest to the homologous gene sequence of Lachnoanaerobaculum orale N1^T (98.5%) and Lachnoanaerobaculum saburreum CCUG 28089^T (97.6%). The major fatty acids of strain ChDC B114^T were C_16:0 (30.7%), C_14:0 (17.7%), iso-C_19:0 (14.9%), and C_17:0 2OH (12.0%). The draft genome of strain ChDC B114^T was 3,097,953 bp in length. The G+C content of the strain was 35.9 mol %. Average nucleotide identity values between strain ChDC B114^T and L. orale N1^T and L. saburreum CCUG 28089^T were 83.2% and 82.0%, respectively. Genome-to-genome distance values between strain ChDC B114^T and L. orale N1^T and L. saburreum CCUG 28089^T were 26.8% (24.5-29.3%) and 26.30% (24.0-28.8%), respectively. Based on these results, strain ChDC B114^T (= KCOM 2030^T = JCM 33452^T) should be classified as a novel species of genus Lachnoanaerobaculum, for which the name Lachnoanaerobaculum gingivalis sp. nov. is proposed.