A male accessory gland peptide that regulates reproductive behavior of female D. melanogaster

Cell. 1988 Jul 29;54(3):291-8. doi: 10.1016/0092-8674(88)90192-4.


The adult male accessory glands of D. melanogaster synthesize and secrete a peptide that represses female sexual receptivity and stimulates oviposition. Normally, this peptide is transferred to females during copulation; however, the peptide shows the same biological activity after purification and subsequent injection into the abdominal cavity of female virgins. Amino acid sequencing of the purified peptide and oligonucleotide-directed cDNA cloning established that the peptide consists of 36 amino acids. It appears to be synthesized as a precursor with a hydrophobic signal sequence of 19 residues at its N-terminal end. The precursor peptide is encoded by a short mRNA that accumulates exclusively in the male accessory gland. The gene has been localized by in situ hybridization to polytene chromosomes at 70A.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Chromatography, High Pressure Liquid
  • Cloning, Molecular
  • DNA / genetics
  • Drosophila Proteins*
  • Drosophila melanogaster / physiology*
  • Female
  • Gene Expression Regulation
  • Intercellular Signaling Peptides and Proteins
  • Male
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Oviposition / drug effects*
  • Ovulation / drug effects
  • Peptide Biosynthesis
  • Peptides / genetics
  • Peptides / isolation & purification*
  • Peptides / pharmacology
  • RNA, Messenger / genetics
  • Sexual Behavior, Animal / drug effects


  • Acp36DE protein, Drosophila
  • Drosophila Proteins
  • Intercellular Signaling Peptides and Proteins
  • Peptides
  • RNA, Messenger
  • SP protein, Drosophila
  • male accessory gland peptide, Drosophila
  • DNA