p55 IL-2 receptor mRNA precursors in murine T lymphocyte nuclei

J Immunol. 1988 Aug 15;141(4):1358-64.


An unusual family of cDNA clones homologous to human p55 IL-2R sequences was isolated from the murine HT-2 Th cell line. These clones were mapped, partially sequenced, and compared with previously published human and mouse IL-2R sequences. They appear to consist of various combinations of exons and introns, suggesting that they are derived from p55 IL-2R mRNA precursors. The configuration of exons in the splicing intermediates indicates that the murine and human gene organizations are similar and that the 3' end of intron 3 is well conserved between the two species. RNA mapping experiments using nuclear, cytoplasmic, and total RNA and probes derived from various parts of the p55 IL-2R gene support and extend the sequence data. They indicate that detectable amounts of immature p55 IL-2R mRNA are found specifically in the cell nucleus of the HT-2 cell line. Similar data were obtained for the Th cell clone 52.3 and the cytotoxic T cell line CTLL. All these results indicate that the T cell nucleus contains significant amounts of immature p55 IL-2R mRNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cell Nucleus / analysis
  • Cloning, Molecular
  • Cytoplasm / analysis
  • Interleukin-2 / metabolism*
  • Mice
  • Molecular Sequence Data
  • Molecular Weight
  • Nucleotide Mapping
  • RNA Precursors / isolation & purification*
  • RNA, Messenger / isolation & purification*
  • Receptors, Immunologic / genetics*
  • Receptors, Interleukin-2
  • Ribonucleases
  • T-Lymphocytes / analysis*


  • Interleukin-2
  • RNA Precursors
  • RNA, Messenger
  • Receptors, Immunologic
  • Receptors, Interleukin-2
  • Ribonucleases

Associated data

  • GENBANK/M21977
  • GENBANK/M21978
  • GENBANK/M21979
  • GENBANK/M21980
  • GENBANK/M21981
  • GENBANK/M21982