Water-soluble polysaccharides from Grifola Frondosa fruiting bodies protect against immunosuppression in cyclophosphamide-induced mice via JAK2/STAT3/SOCS signal transduction pathways

Food Funct. 2019 Aug 1;10(8):4998-5007. doi: 10.1039/c8fo02062k. Epub 2019 Jul 29.


Grifola Frondosa, the king of mushrooms, is one of the most valued traditional medicines and has been used as a health food for a long time in China, Japan, and other Asian countries. The present study was designed to evaluate the immune-modulating effects of water-soluble polysaccharides from the Grifola Frondosa fruiting body (GFP) by using mouse peritoneal macrophage and cytoxan (CTX) induced immunosuppression models. Compared with CTX-induced immunosuppressive mice, the spleen and thymus indexes in mice with GFP orally administrated were significantly increased, body weight loss was alleviated, and the natural killer (NK) cytotoxicity and the proliferative activities of lymphocytes were elevated. Furthermore, levels of interleukin-2 (IL-2), interferon-6 (IL-6) and tumor necrosis factor-α (TNF-α) were notably reduced by CTX, while GFP abolished these effects. GFP also effectively increased total antioxidant capacity and superoxidase dismutase, catalase and glutathione peroxidase activities, and inhibited an increase in the malondialdehyde level. Histopathological analysis of spleens revealed the protective effect of GFP against CTX-induced immunosuppression. Western blotting results showed that GFP possessed immunomodulatory activity by up-regulating transcription factors (p-JAK2/JAK2, p-STAT3/STAT3 and SOCS3) in JAK2/STAT3/SOCS signaling pathways. This study suggested that GFP may provide an alternative strategy for lessening chemotherapy-induced immunosuppression.

MeSH terms

  • Animals
  • Antineoplastic Agents, Alkylating / adverse effects*
  • Cyclophosphamide / adverse effects*
  • Female
  • Grifola / chemistry*
  • Humans
  • Immune System Diseases / drug therapy*
  • Immune System Diseases / etiology
  • Immune System Diseases / genetics
  • Immune System Diseases / immunology
  • Immunosuppression Therapy
  • Interleukin-2 / genetics
  • Interleukin-2 / immunology
  • Janus Kinase 2 / genetics
  • Janus Kinase 2 / immunology*
  • Killer Cells, Natural / drug effects
  • Killer Cells, Natural / immunology
  • Mice
  • Mice, Inbred BALB C
  • Polysaccharides / administration & dosage*
  • Protective Agents / administration & dosage*
  • STAT3 Transcription Factor / genetics
  • STAT3 Transcription Factor / immunology*
  • Signal Transduction / drug effects
  • Suppressor of Cytokine Signaling 3 Protein / genetics
  • Suppressor of Cytokine Signaling 3 Protein / immunology*


  • Antineoplastic Agents, Alkylating
  • Interleukin-2
  • Polysaccharides
  • Protective Agents
  • STAT3 Transcription Factor
  • Socs3 protein, mouse
  • Stat3 protein, mouse
  • Suppressor of Cytokine Signaling 3 Protein
  • Cyclophosphamide
  • Jak2 protein, mouse
  • Janus Kinase 2