Phosphoproteomics Reveals the Biosynthesis of Secondary Metabolites in Catharanthus roseus under Ultraviolet-B Radiation

J Proteome Res. 2019 Sep 6;18(9):3328-3341. doi: 10.1021/acs.jproteome.9b00267. Epub 2019 Aug 7.


Ultraviolet (UV)-B radiation acts as an elicitor to enhance the production of secondary metabolites in medicinal plants. To investigate the mechanisms, which lead to secondary metabolites in Catharanthus roseus under UVB radiation, a phosphoproteomic technique was used. ATP content increased in the leaves of C. roseus under UVB radiation. Phosphoproteins related to calcium such as calmodulin, calcium-dependent kinase, and heat shock proteins increased. Phosphoproteins related to protein synthesis/modification/degradation and signaling intensively changed. Metabolomic analysis indicated that the metabolites classified with pentoses, aromatic amino acids, and phenylpropanoids accumulated under UVB radiation. Phosphoproteomic and immunoblot analyses indicated that proteins related to glycolysis and the reactive-oxygen species scavenging system were changed under UVB radiation. These results suggest that UVB radiation activates the calcium-related pathway and reactive-oxygen species scavenging system in C. roseus. These changes lead to the upregulation of proteins, which are responsible for the redox reactions in secondary metabolism and are important for the accumulation of secondary metabolites in C. roseus under UVB radiation.

Keywords: phosphoproteomics; photosynthesis; secondary metabolites; ultraviolet B.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calcium / metabolism
  • Calmodulin / genetics
  • Calmodulin / metabolism
  • Catharanthus / genetics
  • Catharanthus / metabolism*
  • Catharanthus / radiation effects
  • Phosphoproteins / genetics*
  • Phosphoproteins / radiation effects
  • Plant Leaves / metabolism
  • Plant Leaves / radiation effects
  • Plant Proteins / metabolism*
  • Plant Proteins / radiation effects
  • Plant Roots / metabolism
  • Plant Roots / radiation effects
  • Plants, Medicinal / radiation effects
  • Secondary Metabolism / genetics
  • Secondary Metabolism / radiation effects*
  • Signal Transduction / radiation effects
  • Ultraviolet Rays


  • Calmodulin
  • Phosphoproteins
  • Plant Proteins
  • Calcium