INNO-LiPA DNA line probe assay misidentification of M. smegmatis as Mycobacterium fortuitum complex

Diagn Microbiol Infect Dis. 2019 Nov;95(3):114858. doi: 10.1016/j.diagmicrobio.2019.06.010. Epub 2019 Jun 24.


Seven weeks after being kicked in the face by a cow, a 34-year-old male patient developed a posttraumatic mycobacterial lymphadenitis. A rapidly growing mycobacterial isolate cultured from a surgically drained lymphadenitis pus specimen was identified as Mycobacterium smegmatis by matrix-assisted laser desorption/ionization mass spectrometry and a combination of ITS-, hsp65-, and 16S rRNA-DNA sequence analysis, but as Mycobacterium fortuitum complex using the commercial INNO-LiPA Mycobacteria v2 line probe assay. As it is unclear if the misidentification of this strain is an exception, more research is required.

Keywords: Case report; HSP65 sequencing; INNO-LiPA; Mycobacterium fortuitum complex; Mycobacterium smegmatis.

Publication types

  • Case Reports

MeSH terms

  • Adult
  • Animals
  • Cattle
  • Diagnostic Errors
  • Humans
  • Lymphadenitis / diagnosis*
  • Lymphadenitis / microbiology
  • Lymphadenitis / pathology
  • Lymphadenitis / therapy
  • Male
  • Microbial Sensitivity Tests
  • Molecular Diagnostic Techniques / methods*
  • Molecular Diagnostic Techniques / standards
  • Mycobacterium Infections, Nontuberculous / diagnosis*
  • Mycobacterium Infections, Nontuberculous / microbiology
  • Mycobacterium Infections, Nontuberculous / pathology
  • Mycobacterium Infections, Nontuberculous / surgery
  • Mycobacterium fortuitum / chemistry
  • Mycobacterium fortuitum / classification*
  • Mycobacterium fortuitum / genetics*
  • Mycobacterium smegmatis / chemistry
  • Mycobacterium smegmatis / classification*
  • Mycobacterium smegmatis / genetics*
  • Reagent Kits, Diagnostic
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Treatment Outcome


  • Reagent Kits, Diagnostic