Enterotype-based Analysis of Gut Microbiota along the Conventional Adenoma-Carcinoma Colorectal Cancer Pathway

Sci Rep. 2019 Jul 29;9(1):10923. doi: 10.1038/s41598-019-45588-z.


The dysbiosis of human gut microbiota is strongly associated with the development of colorectal cancer (CRC). The dysbiotic features of the transition from advanced polyp to early-stage CRC are largely unknown. We performed a 16S rRNA gene sequencing and enterotype-based gut microbiota analysis study. In addition to Bacteroides- and Prevotella-dominated enterotypes, we identified an Escherichia-dominated enterotype. We found that the dysbiotic features of CRC were dissimilar in overall samples and especially Escherichia-dominated enterotype. Besides a higher abundance of Fusobacterium, Enterococcus, and Aeromonas in all CRC faecal microbiota, we found that the most notable characteristic of CRC faecal microbiota was a decreased abundance of potential beneficial butyrate-producing bacteria. Notably, Oscillospira was depleted in the transition from advanced adenoma to stage 0 CRC, whereas Haemophilus was depleted in the transition from stage 0 to early-stage CRC. We further identified 7 different CAGs by analysing bacterial clusters. The abundance of microbiota in cluster 3 significantly increased in the CRC group, whereas that of cluster 5 decreased. The abundance of both cluster 5 and cluster 7 decreased in the Escherichia-dominated enterotype of the CRC group. We present the first enterotype-based faecal microbiota analysis. The gut microbiota of colorectal neoplasms can be influenced by its enterotype.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoma / microbiology*
  • Adenoma / pathology
  • Aeromonas / genetics
  • Aeromonas / pathogenicity
  • Aged
  • Bacteroidaceae / genetics
  • Bacteroidaceae / pathogenicity
  • Colorectal Neoplasms / microbiology*
  • Colorectal Neoplasms / pathology
  • Enterococcus / genetics
  • Enterococcus / pathogenicity
  • Escherichia / genetics
  • Escherichia / pathogenicity
  • Female
  • Fusobacterium / genetics
  • Fusobacterium / pathogenicity
  • Gastrointestinal Microbiome*
  • Haemophilus / genetics
  • Haemophilus / pathogenicity
  • Humans
  • Male
  • Middle Aged
  • RNA, Ribosomal, 16S / genetics


  • RNA, Ribosomal, 16S