Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Jul 31;20(1):469.
doi: 10.1186/s13063-019-3557-3.

The Redox-Dependent Regulation of Satellite Cells Following Aseptic Muscle Trauma (SpEED): Study Protocol for a Randomized Controlled Trial

Affiliations
Free PMC article

The Redox-Dependent Regulation of Satellite Cells Following Aseptic Muscle Trauma (SpEED): Study Protocol for a Randomized Controlled Trial

Konstantinos Papanikolaou et al. Trials. .
Free PMC article

Abstract

Background: Muscle satellite cells (SCs) are crucial for muscle regeneration following muscle trauma. Acute skeletal muscle damage results in inflammation and the production of reactive oxygen species (ROS) which may be implicated in SCs activation. Protection of these cells from oxidative damage is essential to ensure sufficient muscle regeneration. The aim of this study is to determine whether SCs activity under conditions of aseptic skeletal muscle trauma induced by exercise is redox-dependent.

Methods/design: Based on the SCs content in their vastus lateralis skeletal muscle, participants will be classified as either high or low respondents. In a randomized, double-blind, crossover, repeated-measures design, participants will then receive either placebo or N-acetylcysteine (alters redox potential in muscle) during a preliminary 7-day loading phase, and for eight consecutive days following a single bout of intense muscle-damaging exercise. In both trials, blood samples and muscle biopsies will be collected, and muscle performance and soreness will be measured at baseline, pre-exercise, 2 and 8 days post exercise. Biological samples will be analyzed for redox status and SCs activity. Between trials, a 4-week washout period will be implemented.

Discussion: This study is designed to investigate the impact of redox status on SCs mobilization and thus skeletal muscle potential for regeneration under conditions of aseptic inflammation induced by exercise. Findings of this trial should provide insight into (1) molecular pathways involved in SCs recruitment and muscle healing under conditions of aseptic skeletal muscle trauma present in numerous catabolic conditions and (2) whether skeletal muscle's potential for regeneration depends on its basal SCs content.

Trial registration: ClinicalTrials.gov, ID: NCT03711838 . Registered on 19 Oct 2018.

Keywords: Antioxidants; Cell signaling; Muscle stem cells; Redox potential; Tissue regeneration.

Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
The Consolidated Standards of Reporting Trials (CONSORT) diagram of the study
Fig. 2
Fig. 2
The experimental flowchart for the clinical trials

Similar articles

See all similar articles

References

    1. Umnova MM, Seene TP. The effect of increased functional load on the activation of satellite cells in the skeletal muscle of adult rats. Int J Sports Med. 1991;12:501–504. doi: 10.1055/s-2007-1024723. - DOI - PubMed
    1. Kadi F, Johansson F, Johansson R, Sjostrom M, Henriksson J. Effects of one bout of endurance exercise on the expression of myogenin in human quadriceps muscle. Histochem Cell Biol. 2004;121:329–334. doi: 10.1007/s00418-004-0630-z. - DOI - PubMed
    1. Le Grand F, Rudnicki MA. Skeletal muscle satellite cells and adult myogenesis. Curr Opin Cell Biol. 2007;19:628–633. doi: 10.1016/j.ceb.2007.09.012. - DOI - PMC - PubMed
    1. Almeida CF, Fernandes SA, Ribeiro Junior AF, Keith Okamoto O, Vainzof M. Muscle satellite cells: exploring the basic biology to rule them. Stem Cells Int. 2016;2016:1078686. doi: 10.1155/2016/1078686. - DOI - PMC - PubMed
    1. Qaisar R, Bhaskaran S, Van Remmen H. Muscle fiber type diversification during exercise and regeneration. Free Radic Biol Med. 2016;98:56–67. doi: 10.1016/j.freeradbiomed.2016.03.025. - DOI - PubMed

Publication types

MeSH terms

Associated data

Feedback