Cholecystokinin inhibits gastric motility and emptying via a capsaicin-sensitive vagal pathway in rats

Am J Physiol. 1988 Aug;255(2 Pt 1):G242-6. doi: 10.1152/ajpgi.1988.255.2.G242.


The pathway by which cholecystokinin octapeptide (CCK-8) inhibits motility of the proximal stomach and the role of this pathway in the CCK-induced delay in gastric emptying of a liquid meal has been studied in rats by selective destruction of vagal afferent C-fibers using bilateral perineural application of the sensory neurotoxin, capsaicin, 3 or 4 days prior to the experiment. The capsaicin treatment significantly attenuated the decrease in intragastric pressure in urethan-anesthetized rats in response to CCK-8 (0.1-100 pmol iv) compared with vehicle-treated controls. Removal of the celiac-superior mesenteric ganglion completely abolished the inhibitory action of CCK-8 on gastric motility in these rats. In contrast, only celiac ganglionectomy in combination with vagotomy abolished the CCK-8 effect in vehicle-treated controls. Intravenous injection of CCK-8 (300 pmol) 5 min before intragastric administration of a methylcellulose solution decreased gastric emptying by 55% in conscious control or vehicle-treated rats. Perivagal capsaicin treatment abolished the delay in gastric emptying induced by CCK-8. In addition, capsaicin treatment alone significantly increased gastric emptying. These results demonstrate that CCK-8 decreases gastric motility in the gastric corpus and delays gastric emptying by a capsaicin-sensitive vagal afferent pathway. These same afferent fibers may also play a physiological role in the gastric emptying of liquids.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Atropine / pharmacology
  • Capsaicin / pharmacology*
  • Gastric Emptying / drug effects*
  • Gastrointestinal Motility / drug effects*
  • Pressure
  • Rats
  • Reference Values
  • Sincalide / pharmacology*
  • Stomach / innervation
  • Stomach / physiology*
  • Thyrotropin-Releasing Hormone / pharmacology
  • Vagotomy
  • Vagus Nerve / drug effects
  • Vagus Nerve / physiology*


  • Thyrotropin-Releasing Hormone
  • Atropine
  • Sincalide
  • Capsaicin