Generalized seizures deplete brain energy reserves in normoxemic newborn monkeys

Brain Res. 1988 Jun 28;454(1-2):51-9. doi: 10.1016/0006-8993(88)90802-5.


The cerebral metabolic response to bicuculline (BC)-induced status epilepticus (SE) was studied in two-week-old ketamine-anesthetized marmoset monkeys. During 30-min clonic seizures, mean blood pressure, plasma glucose and paO2 did not decrease and plasma lactate doubled. Brains were funnel-frozen and punch biopsies of frontoparietal cortex, temporal cortex and thalamus were analyzed for ATP, phosphocreatine (PCr), glucose and lactate. There were marked reductions of ATP (to 56-77% of controls), PCr (to 23-28% of controls) and glucose (to 1-4% of controls), and lactate increased 3- to 6-fold in seizure animals. NADH fluorescence increased during seizures in cerebral cortex, thalamus, amygdaloid nuclei, hippocampus, posterior striatum and hemispheric white matter. This suggests a reduced tissue redox state in these regions and is correlated with the high energy phosphate depletion and elevated lactate in cortex and thalamus. Our results demonstrate a significant depletion of energy reserves and glucose in cerebral cortex and thalamus during neonatal seizures in the absence of adverse systemic factors. These seizure-induced metabolic changes in brain could have adverse long-term effects on brain development and function.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Animals
  • Animals, Newborn / physiology*
  • Bicuculline
  • Brain / metabolism*
  • Brain / physiopathology
  • Callitrichinae / physiology*
  • Energy Metabolism*
  • Glucose / metabolism
  • Lactates / metabolism
  • Lactic Acid
  • NAD / metabolism
  • Phosphocreatine / metabolism
  • Seizures / chemically induced
  • Seizures / metabolism*


  • Lactates
  • Phosphocreatine
  • NAD
  • Lactic Acid
  • Adenosine Triphosphate
  • Glucose
  • Bicuculline