Down-regulation of long noncoding RNA PVT1 inhibits esophageal carcinoma cell migration and invasion and promotes cell apoptosis via microRNA-145-mediated inhibition of FSCN1

Mol Oncol. 2019 Dec;13(12):2554-2573. doi: 10.1002/1878-0261.12555. Epub 2019 Sep 8.


Accumulating evidence has established that long noncoding RNA (lncRNA) plasmacytoma variant translocation 1 (PVT1) is a tumor regulator in many cancers. Here, we aimed to investigate the possible function of lncRNA PVT1 in esophageal carcinoma (EC) via targeting of microRNA-145 (miR-145). Initially, microarray-based gene expression profiling of EC was employed to identify differentially expressed genes. Moreover, the expression of lncRNA PVT1 was examined and the cell line presenting with the highest level of lncRNA PVT1 expression was selected for subsequent experiments. We then proceeded to examine interaction among lncRNA PVT1, FSCN1, and miR-145. The effect of lncRNA PVT1 on viability, migration, invasion, apoptosis, and tumorigenesis of transfected cells was examined with gain-of-function and loss-of-function experiments. We observed that lncRNA PVT1 was robustly induced in EC. lncRNA PVT1 could bind to miR-145 and regulate its expression, and FSCN1 is a target gene of miR-145. Overexpression of miR-145 or silencing of lncRNA PVT1 was revealed to suppress cell viability, migration, and invasion abilities, while also stimulating cell apoptosis. Furthermore, our in vivo results showed that overexpression of miR-145 or silencing of lncRNA PVT1 resulted in decreased tumor growth in nude mice. In conclusion, our research reveals that down-regulation of lncRNA PVT1 could potentially promote expression of miR-145 to repress cell migration and invasion, and promote cell apoptosis through the inhibition of FSCN1. This highlights the potential of lncRNA PVT1 as a therapeutic target for EC treatment.

Keywords: FSCN1; apoptosis; esophageal carcinoma; long noncoding RNA; microRNA-145; plasmacytoma variant translocation 1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Animals
  • Apoptosis*
  • Carrier Proteins* / biosynthesis
  • Carrier Proteins* / genetics
  • Cell Line, Tumor
  • Cell Movement*
  • Down-Regulation*
  • Esophageal Neoplasms* / genetics
  • Esophageal Neoplasms* / metabolism
  • Esophageal Neoplasms* / pathology
  • Female
  • Gene Expression Regulation, Neoplastic
  • Heterografts
  • Humans
  • Male
  • Mice
  • Mice, Nude
  • MicroRNAs* / biosynthesis
  • MicroRNAs* / genetics
  • Microfilament Proteins* / biosynthesis
  • Microfilament Proteins* / genetics
  • Middle Aged
  • Neoplasm Invasiveness
  • Neoplasm Proteins* / biosynthesis
  • Neoplasm Proteins* / genetics
  • Neoplasm Transplantation
  • RNA, Long Noncoding* / biosynthesis
  • RNA, Long Noncoding* / genetics
  • RNA, Neoplasm* / biosynthesis
  • RNA, Neoplasm* / genetics


  • Carrier Proteins
  • FSCN1 protein, human
  • MIRN145 microRNA, human
  • MicroRNAs
  • Microfilament Proteins
  • Neoplasm Proteins
  • PVT1 long-non-coding RNA, human
  • RNA, Long Noncoding
  • RNA, Neoplasm