The Dps4 from Nostoc punctiforme ATCC 29133 is a member of His-type FOC containing Dps protein class that can be broadly found among cyanobacteria

PLoS One. 2019 Aug 1;14(8):e0218300. doi: 10.1371/journal.pone.0218300. eCollection 2019.

Abstract

Dps proteins (DNA-binding proteins from starved cells) have been found to detoxify H2O2. At their catalytic centers, the ferroxidase center (FOC), Dps proteins utilize Fe2+ to reduce H2O2 and therefore play an essential role in the protection against oxidative stress and maintaining iron homeostasis. Whereas most bacteria accommodate one or two Dps, there are five different Dps proteins in Nostoc punctiforme, a phototrophic and filamentous cyanobacterium. This uncommonly high number of Dps proteins implies a sophisticated machinery for maintaining complex iron homeostasis and for protection against oxidative stress. Functional analyses and structural information on cyanobacterial Dps proteins are rare, but essential for understanding the function of each of the NpDps proteins. In this study, we present the crystal structure of NpDps4 in its metal-free, iron- and zinc-bound forms. The FOC coordinates either two iron atoms or one zinc atom. Spectroscopic analyses revealed that NpDps4 could oxidize Fe2+ utilizing O2, but no evidence for its use of the oxidant H2O2 could be found. We identified Zn2+ to be an effective inhibitor of the O2-mediated Fe2+ oxidation in NpDps4. NpDps4 exhibits a FOC that is very different from canonical Dps, but structurally similar to the atypical one from DpsA of Thermosynechococcus elongatus. Sequence comparisons among Dps protein homologs to NpDps4 within the cyanobacterial phylum led us to classify a novel FOC class: the His-type FOC. The features of this special FOC have not been identified in Dps proteins from other bacterial phyla and it might be unique to cyanobacterial Dps proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / metabolism*
  • Ceruloplasmin / chemistry
  • Ceruloplasmin / metabolism*
  • Crystallography, X-Ray
  • DNA-Binding Proteins / chemistry*
  • DNA-Binding Proteins / metabolism*
  • Iron / metabolism*
  • Models, Molecular
  • Nostoc / growth & development
  • Nostoc / metabolism*
  • Oxidation-Reduction
  • Oxidative Stress
  • Protein Conformation
  • Protein Multimerization
  • Sequence Homology
  • Zinc / metabolism*

Substances

  • Bacterial Proteins
  • DNA-Binding Proteins
  • DPS protein, Bacteria
  • Iron
  • Ceruloplasmin
  • Zinc

Grant support

This work was supported by KS: NordForsk (project # 82845) (https://www.nordforsk.org/en), the NCoE program “NordAqua”, KS: Swedish Energy Agency (https://www.energimyndigheten.se/en/), (project # 11674-5); VKM: Post Doc scholarship, Sven and Lilly Lawski foundation, CH: Stiftelsen Lars Hiertas Minne. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.