Indirect Labeling of DNA for Microarrays

Cold Spring Harb Protoc. 2019 Aug 1;2019(8). doi: 10.1101/pdb.prot096461.

Abstract

As with RNA labeling protocols, the main difference between direct and indirect DNA labeling protocols is a trade-off of cost and time. Indirect labeling of DNA, described here, takes ∼2 h longer than direct labeling but is hundreds of dollars cheaper.

MeSH terms

  • Carbocyanines
  • DNA*
  • Fluorescent Dyes
  • Microarray Analysis*
  • Staining and Labeling / methods*

Substances

  • Carbocyanines
  • Fluorescent Dyes
  • DNA