Noninvasive Diagnosis of Infection Using Plasma Next-Generation Sequencing: A Single-Center Experience

Jenna Rossoff; Sonali Chaudhury; Maulin Soneji; Sameer J Patel; Soyang Kwon; Amy Armstrong; William J Muller

doi:10.1093/ofid/ofz327

Noninvasive Diagnosis of Infection Using Plasma Next-Generation Sequencing: A Single-Center Experience

Open Forum Infect Dis. 2019 Aug 1;6(8):ofz327. doi: 10.1093/ofid/ofz327.

Authors

Jenna Rossoff^{1

2}, Sonali Chaudhury^{1

2}, Maulin Soneji^{3

2}, Sameer J Patel^{3

2}, Soyang Kwon^{4

2}, Amy Armstrong^{1

2}, William J Muller^{3

2}

Affiliations

¹ Division of Hematology, Oncology and Transplantation, Ann & Robert H. Lurie Children's Hospital of Chicago, Chicago, Illinois.
² Department of Pediatrics, Northwestern University Feinberg School of Medicine, Chicago, Illinois.
³ Division of Infectious Diseases, Ann & Robert H. Lurie Children's Hospital of Chicago, Chicago, Illinois.
⁴ Stanley Manne Children's Research Institute, Ann & Robert H. Lurie Children's Hospital of Chicago, Chicago, Illinois.

Abstract

Pediatric patients frequently present with illnesses strongly suggesting infection, but without a clearly identified etiology. Our center has recently added a commercially available plasma metagenomic sequencing assay to its available diagnostic testing. Our experience with the first 100 tests suggests that this technology has good clinical performance with >90% sensitivity.

Keywords: bacterial infection; metagenomic next-generation sequencing; viral infection.