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, 2019, 1269534
eCollection

Antimicrobial Activity of an Amnion-Chorion Membrane to Oral Microbes

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Antimicrobial Activity of an Amnion-Chorion Membrane to Oral Microbes

Haroon Ashraf et al. Int J Dent.

Abstract

Objective: The aim of this study was to evaluate wound biomodification by assessing antimicrobial properties present within a human-derived composite amnion-chorion membrane (ACM).

Methods: Membranes analyzed were the human-derived ACM BioXclude™ and the porcine-derived collagen membrane Bio-Gide®. Paper discs with and without tetracycline served as positive and negative controls, respectively. The same number of colony-forming units per milliliter for each bacterial species (Aggregatibacter actinomycetemcomitans, Streptococcus mutans, and Streptococcus oralis) was inoculated on each of the discs. Discs from each group were removed at 12 and 24 hours and sonicated to remove the bacteria off the membranes. A serial dilution was performed to quantify bacterial growth.

Results: The ACM inhibited growth at all time points, with all bacterial strains, identical to the negative control tetracycline discs. The collagen membrane and positive controls did not inhibit growth of any of the bacterial species throughout the 24-hour study period. P < 0.05 for microbial growth on ACM or negative control vs. either collagen membrane or positive control.

Conclusion: ACM was proven to be as bactericidal as paper discs inoculated with tetracycline at its minimum bactericidal concentration. The ACM bactericidal property may be beneficial in the early wound healing process.

Figures

Figure 1
Figure 1
Growth curves for each bacterial species used in the study.
Figure 2
Figure 2
(a) Example of initial inoculum of isolated S.o. in BHI medium. (b) Inoculation of bacteria onto collagen disc. (c) Example of inoculated discs ready for the incubation period (collagen membrane, ACM, negative control, and positive TCN control from left to right). (d) Sonication of bacteria from disc into PBS solution.
Figure 3
Figure 3
(a) Bacterial growth following removal of attempted S.o. culture from the collagen membrane (left half of the plate) and negative control (right half of the plate) at 24 hours following serial dilution. (b) Bacterial growth following removal of attempted A.a. culture from the ACM (left half of the plate, including plating of “inoculum”) and collagen membrane (right half of the plate) at 24 hours following serial dilution.
Figure 4
Figure 4
High contrast image of CFU/mL for A.a. culture during a trial on (a) collagen membrane; (b) negative control; (c) ACM; (d) positive control.
Figure 5
Figure 5
Kill curve for each bacteria on each test and control disc: (a) S.o.; (b) S.m.; (c) A.a. Note: tetracycline and ACM curve are superimposed on each other.

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