Phosphate regulation in Pseudomonas aeruginosa: cloning of the alkaline phosphatase gene and identification of phoB- and phoR-like genes

Mol Gen Genet. 1988 Jun;212(3):510-3. doi: 10.1007/BF00330857.


In Pseudomonas aeruginosa, phosphate limitation results in the synthesis of several protein species. We report the cloning of the P. aeruginosa alkaline phosphatase structural gene, phoA, and we show that this gene is regulated normally in Escherichia coli. We have also identified and cloned two P. aeruginosa genes which can complement phoB and phoR mutations in E. coli. This suggests that a pho regulon system similar to that in E. coli may exist in P. aeruginosa, using at least two similar regulatory factors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaline Phosphatase / genetics*
  • Cell Fractionation
  • Cloning, Molecular
  • Cosmids
  • Escherichia coli / genetics
  • Gene Expression Regulation*
  • Genes
  • Genes, Bacterial*
  • Genetic Complementation Test
  • Phosphates / pharmacology*
  • Plasmids
  • Pseudomonas aeruginosa / enzymology
  • Pseudomonas aeruginosa / genetics*


  • Phosphates
  • Alkaline Phosphatase