A transgene containing lacZ inserted into the dystonia locus is expressed in neural tube

Nature. 1988 Sep 29;335(6189):435-7. doi: 10.1038/335435a0.


The site of integration of transgenes in the host genome can affect levels of expression and occasionally confer ectopic patterns of expression on otherwise tissue-specific genes. We describe here a line of mice in which an hsp68-lacZ transgene is expressed in unstressed developing neural tissue and where the transgene insertion has caused a mutation of a neural tissue-specific gene, dystonia musculorum (dt). This coincidence suggests that expression of the hsp68-lacZ construct may be controlled directly by cis-acting regulatory sequences that normally control the developmental expression of the dt gene. Such constructs may serve as useful tools for identifying new tissue-specific enhancers and their associated genes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Central Nervous System / embryology
  • Central Nervous System / metabolism*
  • Crosses, Genetic
  • DNA
  • Dystonia / genetics*
  • Lac Operon*
  • Mice
  • Mice, Transgenic
  • Mutation
  • Regulatory Sequences, Nucleic Acid
  • beta-Galactosidase / biosynthesis
  • beta-Galactosidase / genetics


  • DNA
  • beta-Galactosidase