ANAC032 regulates root growth through the MYB30 gene regulatory network

Sci Rep. 2019 Aug 6;9(1):11358. doi: 10.1038/s41598-019-47822-0.


Reactive oxygen species (ROS) play important roles as root growth regulators. We previously reported a comprehensive transcriptomic atlas, which we named ROS-map, that revealed ROS-responsible genes in Arabidopsis root tips. By using ROS-map, we have characterised an early ROS response key transcription factor, MYB30, as a regulator of root cell elongation under ROS signals. However, there are other ROS-responsible transcription factors which have the potential to regulate root growth. In the present study, we characterised the function of another early ROS-responsible transcription factor, ANAC032, that was selected from ROS-map. Overexpression of ANAC032 fused with the transcriptional activation domain, VP16, inhibited root growth, especially decreasing cell elongation. By transcriptome analysis, we revealed that ANAC032 regulated many stress-responsible genes in the roots. Intriguingly, ANAC032 upregulated MYB30 and its target genes. The upregulation of MYB30 target genes was completely abolished in the ANAC032-VP16x2 OX and ANAC032 estradiol-inducible line in myb30-2 mutants. Moreover, root growth inhibition was alleviated in ANAC032-OX in myb30-2 mutants. Overall, we characterised an upstream transcription factor, ANAC032, of the MYB30 transcriptional cascade which is a key regulator for root cell elongation under ROS signalling.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / genetics*
  • Arabidopsis / growth & development
  • Arabidopsis / metabolism
  • Arabidopsis Proteins / genetics*
  • Arabidopsis Proteins / metabolism*
  • Arabidopsis Proteins / physiology
  • Gene Expression Profiling
  • Gene Expression Regulation, Plant
  • Gene Regulatory Networks*
  • Meristem / genetics*
  • Meristem / growth & development
  • Meristem / metabolism
  • Reactive Oxygen Species / metabolism
  • Signal Transduction
  • Trans-Activators / metabolism*
  • Trans-Activators / physiology
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism
  • Transcription Factors / physiology


  • ANAC032 protein, Arabidopsis
  • Arabidopsis Proteins
  • MYB30 protein, Arabidopsis
  • Reactive Oxygen Species
  • Trans-Activators
  • Transcription Factors