Effects of long-term feeding of rapeseed meal on skeletal muscle transcriptome, production efficiency and meat quality traits in Norwegian Landrace growing-finishing pigs

PLoS One. 2019 Aug 7;14(8):e0220441. doi: 10.1371/journal.pone.0220441. eCollection 2019.

Abstract

This study was performed to investigate the effects of dietary inclusion of 20% rapeseed meal (RSM) as an alternative to soybean meal (SBM) in a three-month feeding experiment with growing finishing pigs. Dietary alteration affected growth performance, several carcass traits and transcriptional responses in the skeletal muscle, but did not affect measured meat quality traits. In general, pigs fed the RSM test diet exhibited reduced growth performance compared to pigs on SBM control diet. Significant transcriptional changes in the skeletal muscle of growing pigs fed RSM diet were likely the consequence of an increased amount of fiber and higher polyunsaturated fatty acids, and presence of bioactive phytochemicals, such as glucosinolates. RNAseq pipeline using Tophat2-Cuffdiff identified 57 upregulated and 63 downregulated genes in RSM compared to SBM pigs. Significantly enriched among downregulated pathways was p53-mediated signalling involved in cellular proliferation, while activation of negative growth regulators (IER5, KLF10, BTG2, KLF11, RETREG1, PRUNE2) in RSM fed pigs provided further evidence for reduced proliferation and increased cellular death, in accordance with the observed reduction in performance traits. Upregulation of well-known metabolic controllers (PDK4, UCP3, ESRRG and ESRRB), involved in energy homeostasis (glucose and lipid metabolism, and mitochondrial function), suggested less available energy and nutrients in RSM pigs. Furthermore, several genes supported more pronounced proteolysis (ABTB1, OTUD1, PADI2, SPP1) and reduced protein synthesis (THBS1, HSF4, AP1S2) in RSM muscle tissue. In parallel, higher levels of NR4A3, PDK4 and FGF21, and a drop in adropin, ELOVL6 and CIDEC/FSP27 indicated increased lipolysis and fatty acid oxidation, reflective of lower dressing percentage. Finally, pigs exposed to RSM showed greater expression level of genes responsive to oxidative stress, indicated by upregulation of GPX1, GPX2, and TXNIP.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animal Feed / standards*
  • Animals
  • Brassica napus*
  • Economics
  • Gene Expression Regulation
  • Growth
  • Meat / standards*
  • Muscle, Skeletal / metabolism*
  • Oxidative Stress / genetics
  • Swine
  • Transcriptome / genetics*

Grant support

Funding for this research was provided by the FeedMileage-Efficient use of Feed Resources for a Sustainable Norwegian Food Production (Research Council of Norway, Oslo, Norway; grant no. 233685/E50), Foods of Norway, Centre for Research-based Innovation (Research Council of Norway; grant no. 237841/030), and SusPig ERA-Net (Research Council of Norway, Oslo, Norway; grant no. 272334/E50) projects.