Fast, Sensitive and Specific Detection of Thailand orthohantavirus and its Variants Using One-Step Real-Time Reverse-Transcription Polymerase Chain Reaction Assay

Viruses. 2019 Aug 6;11(8):718. doi: 10.3390/v11080718.

Abstract

Genetic variants of Thailand orthohantavirus (THAIV) have been recently reported from rodents in South-East Asia and in islands from the South-West part of the Indian Ocean. In order to detect THAIV and its variants, we developed a sensitive and specific real-time RT-PCR targeting the S segment. Our assay was developed in two different RT-PCR systems that gave similar results in terms of sensitivity. Moreover, our results demonstrated a specificity of 100%.

Keywords: Hantavirus; Real-time RT-PCR; Thailand orthohantavirus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Hantavirus Infections / diagnosis*
  • Hantavirus Infections / virology
  • Orthohantavirus / genetics
  • Orthohantavirus / isolation & purification*
  • RNA, Viral / genetics
  • Rats
  • Real-Time Polymerase Chain Reaction*
  • Sensitivity and Specificity
  • Viral Proteins / genetics
  • Virology / methods*

Substances

  • RNA, Viral
  • Viral Proteins

Supplementary concepts

  • Thailand hantavirus