Neuroprotective Effects of Ozone Therapy After Sciatic Nerve Cut Injury

Kurume Med J. 2020 Jan 23;65(4):137-144. doi: 10.2739/kurumemedj.MS654002. Epub 2019 Aug 8.


A number of antioxidants have been used to treat peripheral nerve injury. However, there are few definitive experimental studies of ozone therapy for peripheral nerve cut injury. We aimed to examine the effects of mild level ozone therapy on sciatic nerve regeneration. One hundred adult male Wistar albino rats were randomly divided into four groups: group 1 (n=20) no cut injury or therapy; group 2 (n=20) sham; group 3 (n=30) nerve cut injury, no therapy; group 4 (n=30) nerve cut injury and ozone therapy. Sciatic functional index (SFI) and withdrawal reflex (WDR) were measured for all groups before nerve cut, at postoperative day 1, and at weeks 2, 4, 6 and 8. More myelinated (M) nerve fibers were observed after nerve cut injury in the ozone-therapy group. Significant differences were seen in plasma SOD (superoxide dismutase), CAT (catalase) and GPx (glutathione peroxidase) activities (p<0.05), and significant functional improvement was observed at postoperative weeks 2 and 4 (p<0.05) after ozone treatment. This is the first study conducted for the purpose of examining the effects of ozone therapy on sciatic nerve cut injury.

Keywords: nerve cut injury; ozone; ozone administration; ozone therapy; peripheric nerve.

MeSH terms

  • Animals
  • Catalase / blood
  • Disease Models, Animal
  • Glutathione Peroxidase / blood
  • Male
  • Motor Activity
  • Nerve Regeneration / drug effects*
  • Neuroprotective Agents / pharmacology*
  • Ozone / pharmacology*
  • Pain Threshold
  • Peripheral Nerve Injuries / blood
  • Peripheral Nerve Injuries / drug therapy*
  • Peripheral Nerve Injuries / physiopathology
  • Rats, Wistar
  • Recovery of Function
  • Sciatic Nerve / drug effects*
  • Sciatic Nerve / injuries
  • Sciatic Nerve / metabolism
  • Sciatic Nerve / physiopathology
  • Sciatic Neuropathy / blood
  • Sciatic Neuropathy / drug therapy*
  • Sciatic Neuropathy / physiopathology
  • Superoxide Dismutase / blood


  • Neuroprotective Agents
  • Ozone
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase