The immunoregulatory effect of sulfated Echinacea purpurea polysaccharide on chicken bone marrow-derived dendritic cells

Lili Yao; Lin Bai; Ying Tan; Jiaqi Sun; Qian Qu; Dayou Shi; Shining Guo; Cui Liu

doi:10.1016/j.ijbiomac.2019.08.028

The immunoregulatory effect of sulfated Echinacea purpurea polysaccharide on chicken bone marrow-derived dendritic cells

Int J Biol Macromol. 2019 Oct 15:139:1123-1132. doi: 10.1016/j.ijbiomac.2019.08.028. Epub 2019 Aug 5.

Authors

Lili Yao¹, Lin Bai², Ying Tan³, Jiaqi Sun⁴, Qian Qu⁵, Dayou Shi⁶, Shining Guo⁷, Cui Liu⁸

Affiliations

¹ College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, PR China. Electronic address: yaolili@stu.scau.edu.cn.
² College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, PR China. Electronic address: Whitelin@stu.scau.edu.cn.
³ College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, PR China. Electronic address: TttYyy@stu.scau.edu.cn.
⁴ College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, PR China. Electronic address: sunjiaqiangel@stu.scau.edu.cn.
⁵ College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, PR China. Electronic address: QuQian@stu.scau.edu.cn.
⁶ College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, PR China; Guangdong Technology Research Center for Traditional Chinese Veterinary Medicine and Nature Medicine, Guangzhou 510642, PR China.. Electronic address: shidayou@scau.edu.cn.
⁷ College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, PR China; Guangdong Technology Research Center for Traditional Chinese Veterinary Medicine and Nature Medicine, Guangzhou 510642, PR China.. Electronic address: shining@scau.edu.cn.
⁸ College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, PR China. Electronic address: liuc@scau.edu.cn.

PMID: 31394150
DOI: 10.1016/j.ijbiomac.2019.08.028

Abstract

Echinacea purpurea polysaccharide (EPP) was obtained by modern separation technology and sulfated EPP (sEPP) was prepared by sulfation modification. The immunological effects of EPP and sEPP were compared on chicken bone marrow-derived dendritic cells (chBM-DCs). The results showed that the surface marker expression of CD11c and CD80 was increased after chBM-DCs were cultured with three dosage of sEPP, especially in sEPP_M group. Three dosage of sEPP, EPP_L and LPS could significantly enhance the effects of chBM-DCs on the proliferation of allogenic mixed lymphocytes. After chBM-DCs treatment with EPP or sEPP in vitro, the levels of IL-2 of sEPP_H and EPP_M groups were significantly higher than those of LPS group (P < 0.05). All sEPP and EPP groups could enhance the level of IFN-γ and down-regulated the level of IL-4 and IL-10. Results indicated that both sEPP and EPP had immunoregulatory effects on chBM-DCs, sEPP possessed better immunoregulatory effects as compared with EPP.

Keywords: Echinacea purpurea polysaccharide (EPP); Immunoregulatory effect; Sulfated EPP (sEPP).

MeSH terms

Animals
Bone Marrow Cells / cytology*
Bone Marrow Cells / drug effects*
Bone Marrow Cells / immunology
Bone Marrow Cells / metabolism
Cell Proliferation / drug effects
Chickens
Cytokines / biosynthesis
Dendritic Cells / cytology*
Echinacea / chemistry*
Immunologic Factors / chemistry
Immunologic Factors / pharmacology
Nitric Oxide / metabolism
Phagocytosis / drug effects
Phenotype
Polysaccharides / chemistry*
Polysaccharides / pharmacology*
Sulfates / chemistry*

Substances

Cytokines
Immunologic Factors
Polysaccharides
Sulfates
Nitric Oxide