Micromonospora acroterricola sp. nov., a novel actinobacterium isolated from a high altitude Atacama Desert soil

Int J Syst Evol Microbiol. 2019 Nov;69(11):3426-3436. doi: 10.1099/ijsem.0.003634.


A Micromonospora strain, designated 5R2A7T, isolated from a high altitude Atacama Desert soil was examined by using a polyphasic approach. Strain 5R2A7T was found to have morphological, chemotaxonomic and cultural characteristics typical of members of the genus Micromonospora. The cell wall contains meso- and hydroxy-diaminopimelic acid, the major whole-cell sugars are glucose, ribose and xylose, the predominant menaquinones MK-10(H4), MK-10(H6), MK-10(H8) and MK-9(H6), the major polar lipids diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unknown glycolipid, and the predominant cellular fatty acids iso-C16 : 0, iso-C15 : 0 and 10-methyl C17 : 0. The digital genomic DNA G+C content is 72.3 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain 5R2A7T was closely related to Micromonospora coriariae DSM 44875T (99.8 %) and Micromonospora cremea CR30T (99.7 %), and was separated readily from the latter, its closest phylogenetic neighbour, based on gyrB and multilocus sequence data, by low average nucleotide identity (92.59 %) and in silico DNA-DNA relatedness (51.7 %) values calculated from draft genome assemblies and by a range of chemotaxonomic and phenotypic properties. Consequently, strain 5R2A7T is considered to represent a novel species of Micromonospora for which the name Micromonospora acroterricola sp. nov. is proposed. The type strain is 5R2A7T (=LMG 30755T=CECT 9656T).

Keywords: Atacama Desert soil; Micromonospora acroterricola; phylogeny; polyphasic taxonomy.

MeSH terms

  • Altitude*
  • Bacterial Typing Techniques
  • Base Composition
  • Cell Wall / chemistry
  • Chile
  • DNA, Bacterial / genetics
  • Desert Climate*
  • Diaminopimelic Acid / chemistry
  • Fatty Acids / chemistry
  • Glycolipids / chemistry
  • Micromonospora / classification*
  • Micromonospora / isolation & purification
  • Nucleic Acid Hybridization
  • Phospholipids / chemistry
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Soil Microbiology*
  • Vitamin K 2 / chemistry


  • DNA, Bacterial
  • Fatty Acids
  • Glycolipids
  • Phospholipids
  • RNA, Ribosomal, 16S
  • Vitamin K 2
  • Diaminopimelic Acid