Measurement of anti-double-stranded DNA antibodies in major immunoglobulin classes

J Immunol Methods. 1979;28(1-2):149-62. doi: 10.1016/0022-1759(79)90337-5.


A solid-phase radioimmunoassay for quantitating anti-double-stranded deoxyribonucleic acid antibodies (anti-dsDNA) in IgG, IgM and IgA classes has been devised. A distinct feature of the method is an application of polystyrene tubes coated with poly-L-lysine, through which dsDNA could be bound firmly to a solid phase. Studies on patients' sea as well as normal sera revealed that anti-dsDNA was not qualitatively but quantitatively characteristic of systemic lupus erythematosus (SLE) and that IgG anti-dsDNA levels correlated well with the disease activity.

MeSH terms

  • Antibodies / analysis*
  • Antibodies / metabolism
  • Antibodies, Anti-Idiotypic / metabolism
  • DNA / immunology*
  • Drug Stability
  • Humans
  • Immunoglobulin A
  • Immunoglobulin G
  • Immunoglobulin M
  • Kinetics
  • Lupus Erythematosus, Systemic / blood
  • Micropore Filters
  • Polystyrenes
  • Radioimmunoassay / methods*
  • Rheumatoid Factor / pharmacology


  • Antibodies
  • Antibodies, Anti-Idiotypic
  • Immunoglobulin A
  • Immunoglobulin G
  • Immunoglobulin M
  • Polystyrenes
  • DNA
  • Rheumatoid Factor