Human iPSC Generation from Antigen-Specific T Cells

Methods Mol Biol. 2019;2048:53-57. doi: 10.1007/978-1-4939-9728-2_5.

Abstract

The discovery and development of induced pluripotent stem cells (iPSCs) opened a novel venue for disease modeling, drug discovery, and personalized medicine. Additionally, iPSCs have been utilized for a wide variety of research and clinical applications without immunological and ethical concerns that encounter embryonic stem cells. Adoptive T cell immunotherapy is a form of cellular immunotherapy that involves transfusion of functional T cells. However, this approach requires T cell expansion and the process causes T cell exhaustion. As a result, highly expanded T cells have not proven to be particularly effective for treatments. This exhaustion issue could be overcome due to rejuvenation of T cells by reprogramming to pluripotency and redifferentiation to T cells. This is a potential therapeutic strategy for combating various types of cancer.

Keywords: Cancer immunotherapy; Pluripotent stem cell; T cell.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cellular Reprogramming / genetics
  • Cellular Reprogramming / immunology*
  • Genetic Vectors / genetics
  • Humans
  • Immunotherapy, Adoptive / methods
  • Induced Pluripotent Stem Cells / physiology*
  • Neoplasms / immunology
  • Neoplasms / therapy
  • Primary Cell Culture / methods*
  • Recombinant Proteins / genetics
  • Sendai virus / genetics
  • T-Lymphocytes, Cytotoxic / physiology*
  • Transcription Factors / genetics
  • Transduction, Genetic / methods

Substances

  • Recombinant Proteins
  • Transcription Factors